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The Normal Karyotype 46,XY Reference Standard is an essential tool for validating chromosome analysis workflows in clinical cytogenetics, ensuring accurate detection of numerical and structural chromosomal abnormalities. This reference standard consists of metaphase spreads derived from healthy male donor lymphocytes, meticulously prepared to exhibit clear G-banding patterns across all 22 autosome pairs and the XY sex chromosomes . Its design supports quality control in laboratories performing karyotyping for infertility, prenatal diagnosis, and hematological malignancy screening.
Compliant with ISCN (International System for Human Cytogenomic Nomenclature) standards, this reference standard provides a benchmark for assessing the resolution and accuracy of chromosome banding techniques. Each spread demonstrates the characteristic banding pattern of a normal male karyotype, enabling laboratories to verify their ability to identify subtle chromosomal rearrangements and ensure consistent interpretation across technologists.
• High-Resolution Banding: Exhibits 400-550 bands per haploid set, allowing visualization of small chromosomal segments critical for detecting microdeletions or duplications.
• Morphological Integrity: Metaphase spreads are evenly distributed with minimal overlapping chromosomes, reducing counting errors and improving analysis efficiency.
• Stability: Fixed preparations remain stable for up to 24 months when stored at -20°C, with no degradation in banding quality over time .
• Traceability: Each lot is karyotyped by two independent cytogeneticists, with digital images of representative spreads included for comparison.
Slide Preparation:
• Thaw frozen slides at room temperature for 30 minutes before use, avoiding rapid temperature changes that can cause condensation.
• Stain with Giemsa or Wright’s stain following standard laboratory protocols to enhance G-banding contrast.
Karyotyping Validation:
• Analyze a minimum of 20 metaphase spreads to confirm the presence of 46 chromosomes with a normal male (XY) sex chromosome complement .
• Verify banding clarity by identifying key landmarks, such as the centromeric position of chromosome 1 and the distinctive banding of the Y chromosome.
Quality Control Implementation:
• Include the reference standard in monthly quality control runs to monitor staining consistency and technologist performance.
• Use digital karyotyping software to compare results against the provided reference images, ensuring alignment with ISCN nomenclature.
What is the significance of G-banding resolution in this standard?
The 400-550 band level allows detection of chromosomal abnormalities as small as 5-10 Mb, which is critical for diagnosing conditions like microdeletion syndromes.
Can it be used for training in karyotype interpretation?
Yes. Its clear banding patterns and normal karyotype make it an excellent tool for training staff in chromosome identification and pairing.
How should stained slides be stored long-term?
Store stained slides at room temperature in a dry, dark slide box; avoid exposure to direct sunlight, which can fade banding over time.
Does it include guidelines for karyotype reporting?
Yes. The accompanying manual provides examples of ISCN-compliant reports for normal 46,XY karyotypes, aiding in standardizing laboratory documentation.
Product Information | Normal Karyotype (46,XY) Reference Standard |
Catalog ID | CBPJ0004 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing | Download |
Storage | 2-8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Mutation | Normal Karyotype |
Karyotype | 46,XY |
Representative Data
Z-Score:
chr | chrl | chr2 | chr3 | chr4 | chr5 | chr6 | chr7 | chr8 | chr9 | chr10 | chrll | chr12 | chrl3 | chr14 | chr15 | chr16 | chr17 | chr18 | chr19 | chr20 | chr21 | chr22 |
CBPJ0004 | 1.45 | -2.98 | 0.64 | 0.00 | 1.26 | 1.00 | 1.29 | -1.55 | -1.55 | -2.47 | -0.32 | 1.77 | -0.97 | 0.37 | -2.81 | -0.45 | 1.08 | -1.95 | 2.58 | -1.14 | -1.52 | 0.23 |
The Normal Karyotype 46,XY Reference Standard is an essential tool for validating chromosome analysis workflows in clinical cytogenetics, ensuring accurate detection of numerical and structural chromosomal abnormalities. This reference standard consists of metaphase spreads derived from healthy male donor lymphocytes, meticulously prepared to exhibit clear G-banding patterns across all 22 autosome pairs and the XY sex chromosomes . Its design supports quality control in laboratories performing karyotyping for infertility, prenatal diagnosis, and hematological malignancy screening.
Compliant with ISCN (International System for Human Cytogenomic Nomenclature) standards, this reference standard provides a benchmark for assessing the resolution and accuracy of chromosome banding techniques. Each spread demonstrates the characteristic banding pattern of a normal male karyotype, enabling laboratories to verify their ability to identify subtle chromosomal rearrangements and ensure consistent interpretation across technologists.
• High-Resolution Banding: Exhibits 400-550 bands per haploid set, allowing visualization of small chromosomal segments critical for detecting microdeletions or duplications.
• Morphological Integrity: Metaphase spreads are evenly distributed with minimal overlapping chromosomes, reducing counting errors and improving analysis efficiency.
• Stability: Fixed preparations remain stable for up to 24 months when stored at -20°C, with no degradation in banding quality over time .
• Traceability: Each lot is karyotyped by two independent cytogeneticists, with digital images of representative spreads included for comparison.
Slide Preparation:
• Thaw frozen slides at room temperature for 30 minutes before use, avoiding rapid temperature changes that can cause condensation.
• Stain with Giemsa or Wright’s stain following standard laboratory protocols to enhance G-banding contrast.
Karyotyping Validation:
• Analyze a minimum of 20 metaphase spreads to confirm the presence of 46 chromosomes with a normal male (XY) sex chromosome complement .
• Verify banding clarity by identifying key landmarks, such as the centromeric position of chromosome 1 and the distinctive banding of the Y chromosome.
Quality Control Implementation:
• Include the reference standard in monthly quality control runs to monitor staining consistency and technologist performance.
• Use digital karyotyping software to compare results against the provided reference images, ensuring alignment with ISCN nomenclature.
What is the significance of G-banding resolution in this standard?
The 400-550 band level allows detection of chromosomal abnormalities as small as 5-10 Mb, which is critical for diagnosing conditions like microdeletion syndromes.
Can it be used for training in karyotype interpretation?
Yes. Its clear banding patterns and normal karyotype make it an excellent tool for training staff in chromosome identification and pairing.
How should stained slides be stored long-term?
Store stained slides at room temperature in a dry, dark slide box; avoid exposure to direct sunlight, which can fade banding over time.
Does it include guidelines for karyotype reporting?
Yes. The accompanying manual provides examples of ISCN-compliant reports for normal 46,XY karyotypes, aiding in standardizing laboratory documentation.
Product Information | Normal Karyotype (46,XY) Reference Standard |
Catalog ID | CBPJ0004 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing | Download |
Storage | 2-8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Mutation | Normal Karyotype |
Karyotype | 46,XY |
Representative Data
Z-Score:
chr | chrl | chr2 | chr3 | chr4 | chr5 | chr6 | chr7 | chr8 | chr9 | chr10 | chrll | chr12 | chrl3 | chr14 | chr15 | chr16 | chr17 | chr18 | chr19 | chr20 | chr21 | chr22 |
CBPJ0004 | 1.45 | -2.98 | 0.64 | 0.00 | 1.26 | 1.00 | 1.29 | -1.55 | -1.55 | -2.47 | -0.32 | 1.77 | -0.97 | 0.37 | -2.81 | -0.45 | 1.08 | -1.95 | 2.58 | -1.14 | -1.52 | 0.23 |
