An sgRNA library, short for "single guide RNA library," is a collection of tens to hundreds of thousands of different sgRNA molecules. Each sgRNA acts like a "molecular key," precisely guiding the CRISPR/Cas9 gene editing system to a specific site on the genome for cutting. Therefore, an sgRNA library is essentially a vast "toolbox" that can target virtually any gene, allowing scientists to simultaneously conduct large-scale functional gene screening across the entire genome.

Studying the function of genes in metabolic pathways or in the occurrence of certain diseases is of great significance for the study of disease mechanisms and drug development. The new-generation gene editing technology CRISPR-Cas9 has become a powerful tool for forward genetic screening experiments due to its unique advantages of simple operation and versatility. CRISPR guide sgRNAs are ideal for high-throughput screening of important molecular targets. Use CRISPR-Cas9 technology to establish a mutant library that may be related to a certain type of function, and confirm the genes related to this type of function through functional screening and enrichment, PCR amplification and deep sequencing analysis.
CB-Gene provides customers with one-stop gene function screening services from sgRNA library design, chip Oligo primer synthesis, library construction and lentiviral packaging to screening.