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CRISPR-Cas9 sgRNA library

Definition

An sgRNA library, short for "single guide RNA library," is a collection of tens to hundreds of thousands of different sgRNA molecules. Each sgRNA acts like a "molecular key," precisely guiding the CRISPR/Cas9 gene editing system to a specific site on the genome for cutting. Therefore, an sgRNA library is essentially a vast "toolbox" that can target virtually any gene, allowing scientists to simultaneously conduct large-scale functional gene screening across the entire genome.

Service Background

Investigating gene function in metabolic pathways and disease processes plays a critical role in uncovering disease mechanisms and facilitating therapeutic development. CRISPR-Cas9, as a next-generation gene editing technology, has emerged as a robust platform for forward genetic screening, owing to its simplicity, scalability, and versatility. Genome-wide or targeted sgRNA libraries enable high-throughput interrogation of genes involved in specific biological functions. Through functional screening, enrichment analysis, PCR amplification, and next-generation sequencing, genes associated with the phenotype of interest can be systematically identified.

CB-Gene offers an integrated, end-to-end gene function screening service, encompassing sgRNA library design, oligonucleotide synthesis, library construction, lentiviral packaging, and downstream screening and analysis.

Service Advantage

The entire service workflow strictly adheres to QC standards. The sgRNA plasmid library is maintained at a storage capacity exceeding 500× the total number of sgRNAs, and library coverage is verified by NGS sequencing to achieve over 99% representation..

Lentiviral transduction is employed to maximize sgRNA delivery efficiency and ensure robust gene perturbation.

Rich screening platform: control cell survival, combined with immunostaining, flow cytometry and other screening methods.

Multiple screening approaches are available, including control of cell viability, immunostaining, flow cytometry, and other phenotypic assays, allowing flexible and precise functional screening.