sgRNA library

An sgRNA library, short for "single guide RNA library," is a collection of tens to hundreds of thousands of different sgRNA molecules. Each sgRNA acts like a "molecular key," precisely guiding the CRISPR/Cas9 gene editing system to a specific site on the genome for cutting. Therefore, an sgRNA library is essentially a vast "toolbox" that can target virtually any gene, allowing scientists to simultaneously conduct large-scale functional gene screening across the entire genome.

Investigating gene function in metabolic pathways and disease processes plays a critical role in uncovering disease mechanisms and facilitating therapeutic development. CRISPR-Cas9, as a next-generation gene editing technology, has emerged as a robust platform for forward genetic screening, owing to its simplicity, scalability, and versatility. Genome-wide or targeted sgRNA libraries enable high-throughput interrogation of genes involved in specific biological functions. Through functional screening, enrichment analysis, PCR amplification, and next-generation sequencing, genes associated with the phenotype of interest can be systematically identified.