CRISPR/Cas9 is a revolutionary gene editing tool that uses guide RNA (sgRNA) and Cas9 enzymes to introduce targeted DNA breaks at specific locations in the genome, resulting in gene destruction. It plays an important role in antibody specificity verification, determining drug targets, and determining genes involved in pathways.

An sgRNA library, short for "single guide RNA library," is a collection of tens to hundreds of thousands of different sgRNA molecules. Each sgRNA acts like a "molecular key," precisely guiding the CRISPR/Cas9 gene editing system to a specific site on the genome for cutting.

Off-target effects occur when gene editing tools (such as CRISPR-Cas9) accidentally identify and cleave other similar, but not identical, sequences in the genome while targeting and cleaving a specific target DNA sequence. These unintended cleavages can lead to gene mutations, cellular dysfunction, and even cancer, and are one of the most significant safety concerns in gene therapy and clinical applications.

Material Number: CBP10402

Material Number: CBP10428

Material Number: CBP10402