Stable Cell Line Generation

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Lenti-integrateds

Lentivirus-integrated stable cell line refers to a type of cell line that uses lentivirus as a vector to efficiently introduce a specific exogenous gene (such as a reporter gene, a functional gene or shRNA) into the target cell, and this exogenous gene can be stably inherited to daughter cells as the cell divides and proliferates.

Flp-FRT integrated

The Flp-FRT integration system is a method for generating stable cell lines that express a gene of interest at a specific location in the host cell genome. This system utilizes the Flp recombinase and FRT (Flp Recombination Target) sites to achieve site-specific recombination and integration. This system ensures stable expression of the gene of interest, ensuring continuous protein production.

Plasmid random-integrated

Plasmid transfection is a technique for introducing plasmid DNA into cells, enabling scientists to study gene function, expression, and cellular effects. It involves introducing foreign DNA into cells using non-viral methods, such as electroporation or chemical reagents.

CB-Gene provides a "Plasmid Random Integration System" stable cell line construction service. Using this system, we have successfully constructed more than 500 stable cell line models and have rich experience.

Electroporation-integrated

Electroporation, also known as electropermeabilization, is a highly effective non-viral delivery system for genetic material (DNA and RNA), proteins, drugs, or other molecules into cells. It uses precisely pulsed electric current to create temporary pores in the cell membrane, allowing molecules to pass through.

Once plasmid DNA enters the cell, it can act on the cell. The genetic material may remain isolated (in the form of a plasmid, not integrated) or, depending on subsequent experimental steps, may integrate into the host genome.

Transposon-integrated

A transposon-integrated stable cell line refers to a cell population that utilizes the transposon system as a gene delivery tool to efficiently integrate an exogenous target gene into the host cell genome, where it is stably inherited and expressed as cells divide.

The core of this system lies in the concepts of "transposon" and "integration."