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Stable Cell Line Generation

Lenti-integrateds

Lentivirus is a genus of retroviruses within the Retroviridae family. Lentiviruses encode reverse transcriptase and integrase, enabling viral RNA to be reverse-transcribed into DNA and stably integrated into the host genome. Following integration, the viral genetic material is replicated during host cell division, allowing for long-term and stable gene expression.

Flp-FRT integrated

Flp (flippase) recombinase is a site-specific recombination enzyme derived from yeast. The FLP gene is approximately 1272 bp in length and encodes a 423-amino-acid polypeptide with a molecular weight of approximately 48 kDa. Flp functions as a monomer.

FRT (Flp Recombination Target) is the specific DNA sequence recognized by Flp recombinase. The FRT site is 48 bp in length and consists of two 13-bp inverted repeats flanking an 8-bp asymmetric spacer, along with an additional 13-bp repeat. The inverted repeats serve as Flp binding sites, while the spacer region defines the recombination site and confers directionality to the recombination event.

Plasmid random-integrated

Plasmid transfection is a technique for introducing plasmid DNA into cells, enabling scientists to study gene function, expression, and cellular effects. It involves introducing foreign DNA into cells using non-viral methods, such as electroporation or chemical reagents.

CB-Gene provides stable cell line development services based on a “Plasmid Random Integration System”. To date, we have successfully generated over 500 stable cell line models, demonstrating extensive experience in plasmid-based genome integration.

Electroporation-integrated

Electroporation, also known as electropermeabilization, is a highly efficient non-viral delivery method for introducing nucleic acids (DNA or RNA), proteins, drugs, and other molecules into cells. Short, controlled electrical pulses transiently permeabilize the cell membrane, enabling the uptake of these molecules.

Once plasmid DNA enters the cell, it can act on the cell. The genetic material may remain isolated (in the form of a plasmid, not integrated) or, depending on subsequent experimental steps, may integrate into the host genome.

Transposon-integrated

Transposable elements (TEs), also known as jumping genes, are DNA sequences capable of moving from one genomic location to another. First discovered by Barbara McClintock, TEs are now recognized as ubiquitous components of genomes across both prokaryotes and eukaryotes. In many organisms, they constitute a substantial proportion of the genome—for example, approximately 50% of the human genome and up to 90% of the maize genome.