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RT-PCR Testing Services

Definition

RT-PCR, short for reverse transcription-polymerase chain reaction, is an extremely sensitive and powerful molecular biology technique. It combines the processes of reverse transcription and polymerase chain reaction to detect and quantify trace amounts of specific RNA molecules.

The core purpose is to reverse transcribe RNA into more stable complementary DNA. PCR is then used to exponentially amplify this cDNA, enabling detection, qualitative, and quantitative analysis.

Its core value lies in transforming a complex technology into a standardized, high-quality, and high-efficiency end product, significantly lowering the barrier to entry for users and accelerating research and project timelines.

Working Principle

RT-PCR is essentially a two-stage enzymatic reaction process designed for the qualitative or quantitative analysis of specific RNA sequences present in minute amounts.

Stage 1

Reverse Transcription (RT)

Translating the "Language of RNA" into the "Language of DNA"

The reverse transcription step utilizes reverse transcriptase, which uses the total RNA sample as a template. Guided by specific primers (Oligo(dT) primers to anchor the polyA tail of mRNA, or random hexamers for comprehensive capture), it catalyzes the synthesis of a complementary DNA strand, known as complementary DNA (cDNA).

Stage 2

Quantitative Real-Time PCR (qPCR）

Exponential Amplification and Real-Time Monitoring of the "DNA Instructions"

The cDNA synthesized in the first stage is used as a template. Specific primers, a DNA polymerase (e.g., thermostable Taq polymerase), and a fluorescent reporting system (described below) are added to perform cyclic PCR amplification. Each cycle consists of:Denaturation、Annealing and Extension.

Standardized Workflow

A reliable testing service stems from meticulous process control. Its Standard Operating Procedure (SOP) is visually summarized in the flowchart below, with quality checks integrated into every stage:

Advantages

Exceptional Sensitivity and Specificity

Capable of detecting very low copy numbers (even single copies) of RNA and effectively discriminating between highly homologous sequences.

Precise Quantification

Provides digital results for either absolute quantification (copy number) or relative quantification (expression fold-change), far surpassing traditional qualitative methods.

High Throughput and Efficiency

The 96-well or 384-well plate format allows for the simultaneous processing of a large number of samples, ensuring rapid turnaround.

Mature and Standardized Technology

Decades of development have resulted in robust protocols that are easily standardized and scaled at the service level.

Service Application

Professional RT-PCR testing services have an extremely wide range of applications, covering nearly all life science and related industries:

Basic Scientific Research:

Gene Expression Profiling/Molecular Mechanism Exploration/Non-Coding RNA Research

Medical Diagnostics and Precision Medicine:

Pathogen Nucleic Acid Detection/Cancer Molecular Typing and Prognosis/Genetic Disease Screening and Diagnosis/Drug Discovery and Biomarker Validation

Agriculture and Food Safety:

Genetically Modified Organism (GMO) Detection/Animal and Plant Breeding/Animal and Plant Disease Diagnostics

Forensic Science:

Body Fluid Identification

Professional RT-PCR Testing Services democratize powerful molecular detection capabilities through a specialized and scalable operational model, making them accessible beyond a few elite laboratories. Characterized by their precision, sensitivity, efficiency, and reliability, these services are a powerful engine driving innovation in life sciences, advancing precision medicine, and safeguarding public health.

Choosing CB-Gene equates to choosing reliable data quality, efficient project cycles, and in-depth analytical support, allowing you to focus more on the scientific questions themselves and the expansion of core business. On the path to unraveling the mysteries of life, professional RT-PCR testing services are a trusted ally.

If you are interested in ordering, please contact us
Email: sales@cb-gene.com