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Methylation single gene single segment standard
Product Introduction
Based on the fact that methylation has a clear prospect in the early detection and screening of tumors, CB-Gene Bio also chooses tumor-related gene methylation as the research object. Some genes are shown in the following table:
Table 1: Some genes related to tumors, red ones are relatively hot genes
As shown in the figure, our company launched the first batch of methylation standards for genes such as BMP3/SDC2/SEPTIN9/MGMT/Twist1/RASSF1A/SHOX2
Development ideas
CB-Gene will continue to develop corresponding standards for the above genes. The development ideas are mainly divided into 4 steps:
01
Screening: hotspot gene
According to the sequence of the gene, find the key CGI, combine the structure of the gene transcript, and the relationship with TSS to preliminarily screen out the key CGI;
02
Methylation island
Establish an internal preliminary screening system, combine the selected CGI segment, select amplicons within a certain range, use common methylation detection methods (such as BSP and pyrophosphate sequencing), and detect the methylation data of multiple amplicons in the target region;
03
Small amplicon region
According to the data in step 2, carefully judge the useful small amplicon region, develop dPCR in this region, and detect the methylation degree of the CG;
04
Precise quantification
Based on the methylation data of this region and this site, establish a complete set of reference and quality control product systems.
The above is the idea of CB-Gene Bio's internal development of methylation standards. For more specific gene cases, we will launch our products in the near future
