Customized Standards

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Customized Materials

SNV

SNV standards refer to genomic DNA extracted from cells, rigorously quality-controlled, and standardized with known concentration, sequence information, and specific genetic variant characteristics. They are commonly used to calibrate experimental systems, validate the accuracy of assays, evaluate the performance of sequencing or PCR technologies, and serve as positive controls or quantitative references. Unlike standard SNVs, standards must possess high purity, integrity, and stability, and their genetic background and variant information must be fully verified.

Indel

Indels are small insertions or deletions of DNA sequences at specific locations in the genome. These variants are important contributors to many genetic diseases and cancers. Indel standards are artificially constructed DNA samples containing specific indel variants with known locations, sequences, and frequencies.

It can be thought of as a test question with a known answer. Testing laboratories use technologies such as high-throughput sequencing (NGS) to analyze these reference standards and then compare the results with the reference standard. Through this process, laboratories can comprehensively evaluate the accuracy, sensitivity, specificity, and reproducibility of the entire process, from library construction to sequencing to data analysis, ensuring the most reliable testing for every real clinical sample.

Fusion

A fusion gene occurs when all or part of the sequences of two genes fuse to form a new gene. This can occur as a result of a chromosomal translocation, interstitial deletion, or chromosomal inversion.

If the desired site cannot be found in the natural sample, we can use cell engineering technology to edit specific chromosomal locations. The edited cell line has clear breakpoints and is completely identical to the natural sequence, enabling stable detection at the RNA level and testing the sensitivity, specificity, and stability of fusion gene detection.

Panel

A panel standard refers to a set of purified substances (such as proteins, nucleic acids, and metabolites) that have undergone rigorous qualitative and quantitative validation. They are used to establish calibration curves for analytical methods, verify experimental performance, and ensure the accuracy of results. Unlike a single standard, a panel standard typically contains multiple relevant biomarkers, simulating the actual composition of complex biological samples and providing systematic quality assurance for multiplexed assays (such as mass spectrometry, immunoassays, and sequencing).

IHC

IHC standards are control samples used in immunohistochemistry experiments. They typically contain sections of known positive and negative tissues or cell lines. They are used to verify the reliability of experimental procedures, the specificity of antibodies, and the accuracy of results. They help researchers standardize protocols, ensure consistency across batches, and reduce variability. For example, when testing HER2 expression in breast cancer, standards must clearly indicate positive and negative results to calibrate experimental conditions.