NGS Quality Control Reference Standards

NGS Instrument Validation

When acquiring a new NGS platform or performing routine instrument qualification, our reference standards provide a reliable benchmark. Use them to evaluate sequencing accuracy, read depth uniformity, base calling quality, and reproducibility across multiple runs. For laboratories expanding from one platform to another (e.g., Illumina to MGI Tech), the standards enable direct cross-platform performance comparison, helping you harmonize data quality across different instruments.

Library Preparation Optimization & Reagent Lot Testing

Library preparation is a critical step where variability can significantly impact final data quality. Our reference standards serve as process controls to monitor key library preparation metrics including DNA fragmentation efficiency, adapter ligation consistency, amplification bias, and library yield. When switching to a new batch of library prep reagents or adopting a new kit, run the standard alongside your test samples to confirm that performance meets expected specifications before processing valuable patient samples.

Routine Run QC & Panel Validation

Integrate our NGS reference standards into your daily sequencing runs as in-process quality controls to monitor run-to-run variability and detect early signs of workflow drift. For research-use kit developers, the standards support panel performance validation – test capture efficiency, coverage uniformity, and variant calling accuracy across your target regions. When unexpected results occur, the standards serve as troubleshooting tools to pinpoint whether the issue originates from sample extraction, library prep, sequencing, or bioinformatics analysis, saving time and resources in root cause investigation.