- Home
- Products
- Services
- Resources
- About Us
- News
- Contact
Description
The gDNA Cocktail V Reference Standard contains important sites of common genes, involving 12 gene CNV sites such as EGFR/BRAF/KRAS/PIK3CA/EML4-ALK/CCDC6-RET/ERBB2, covering multiple variation types such as SNP Del Translocation CNV, with a variation frequency of 3%, and dPCR calibration.
Features
1. The samples are derived from clinically isolated cell lines, which are very close to the characteristics of clinical patients；
2. Covers a variety of cancer-related mutation sites: lung cancer, colorectal cancer, breast cancer, gastric cancer, ovarian cancer, pancreatic cancer, etc.
3. dPCR calibration；
4. Contains important sites of common genes, involving 12 gene CNV sites
General information
| Name | Panel-Ref® gDNA Cocktail V Reference Standard |
| Cat. No. | CBP90014 |
| Format | gDNA |
| Size | 1ug/vial * 1 vial |
| Intended Use | Research Use Only |
| Inventory Status | In Stock |
| Buffer | Tris-EDTA |
| Storage Conditions | 2-8℃ |
| Expiry | 36 months from the date of manufacture |
Detailed Data
| Gene | AA Change | CDS Change | Exon | Cosmic ID | Expected %AF | Chr position (GRCh37) |
| EGFR | p.E746_A750delELREA | c.2235_2249del15 GGAATTAAGAGAAGC>- | 19 | COSM6223 | 3.0 | chr7:55242465_55242479 |
| EGFR | p.L858R | c.2573T>G | 21 | COSM6224 | 3.0 | chr7:55259515 |
| EGFR | p.T790M | c.2369C>T | 20 | COSM6240 | 3.0 | chr7:55249071 |
| EGFR | p.G719S | c.2155G>A | 18 | COSM6252 | 3.0 | chr7:55241707 |
| BRAF | p.V600E | c.1799T>A | 15 | COSM476 | Endogenous mutations | chr7:140453136 |
| KRAS | p.G12D | c.35G>A | 2 | COSM521 | 3.0 | chr12:25398284 |
| KRAS | p.Q61H | c.183A>C | 3 | COSM554 | Endogenous mutations | chr12:25380275 |
| PIK3CA | p.E545K | c.1633G>A | 10 | COSM763 | 3.0 | chr3:178936091 |
| PIK3CA | p.H1047R | c.3140A>G | 20 | COSM775 | Endogenous mutations | chr3:178952085 |
| EML4-ALK | E13-A20 | DNA Translocation | N/A | COSF408 | 3.0 | N/A |
| CCDC6-RET | E1-E12 | DNA Translocation | N/A | COSF1271 | 3.0 | N/A |
| ERBB2 | CNV | Amplication | N/A | N/A | CN=4 | N/A |
Product application
1.Nucleic Acid Quantification and Quality Control
2.Gene Copy Number Variation Analysis
3.Genotyping and Mutation Detection:Used for the development and validation of various genotyping assays.
*Positive control: In assays such as ARMS-PCR, Sanger sequencing, and NGS, these standards provide clear comparisons of wild-type and mutant variants (e.g., EGFR L858R, KRAS G12D).
*Determining the limit of detection: By mixing mutant and wild-type standards at a specific ratio, the sensitivity of the assay can be determined. For example, determining whether a low-frequency mutation of 1% can be detected, which is crucial for tumor liquid biopsies.
*Allele Quantification: Used to accurately quantify the ratios of different alleles when analyzing methylation, mosaicism, and other conditions.
4.Next-Generation Sequencing
NGS processes are highly complex, and gDNA standards are central to quality control throughout the entire process.
*Library Construction Quality Control: As the starting material, gDNA standards monitor the efficiency and quality of library construction.
*Inter-Laboratory Comparison: Using the same set of standards allows for comparability of NGS data across different laboratories and platforms.
Related Products List
| Name | Catalog No. | Details |
| Panel-Ref® gDNA Cocktail II Reference Standard | CBP90009 | |
| Panel-Ref® gDNA Cocktail III Reference Standard | CBP90010 | |
| Panel-Ref® gDNA Cocktail IV Reference Standard | CBP90011 | |
| Panel-Ref® gDNA Cocktail VI Reference Standard | CBP90015 | |
| Panel-Ref® gDNA Cocktail Ⅶ Reference Standard | CBP90016 | |
| Panel-Ref® gDNA Cocktail VIII Reference Standard | CBP90020 | |
| Panel-Ref® gDNA Cocktail I Reference Standard | CBP90049 |
