Description

The gDNA Cocktail V Reference Standard contains important sites of common genes, involving 12 gene CNV sites such as EGFR/BRAF/KRAS/PIK3CA/EML4-ALK/CCDC6-RET/ERBB2, covering multiple variation types such as SNP Del Translocation CNV, with a variation frequency of 3%, and dPCR calibration.

Features

1. The samples are derived from clinically isolated cell lines, which are very close to the characteristics of clinical patients；

2. Covers a variety of cancer-related mutation sites: lung cancer, colorectal cancer, breast cancer, gastric cancer, ovarian cancer, pancreatic cancer, etc.

3. dPCR calibration；

4. Contains important sites of common genes, involving 12 gene CNV sites

General information

Name	Panel-Ref® gDNA Cocktail V Reference Standard
Cat. No.	CBP90014
Format	gDNA
Size	1ug/vial * 1 vial
Intended Use	Research Use Only
Inventory Status	In Stock
Buffer	Tris-EDTA
Storage Conditions	2-8℃
Expiry	36 months from the date of manufacture

Detailed Data

Gene	AA Change	CDS Change	Exon	Cosmic ID	Expected %AF	Chr position (GRCh37)
EGFR	p.E746_A750delELREA	c.2235_2249del15 GGAATTAAGAGAAGC>-	19	COSM6223	3.0	chr7:55242465_55242479
EGFR	p.L858R	c.2573T>G	21	COSM6224	3.0	chr7:55259515
EGFR	p.T790M	c.2369C>T	20	COSM6240	3.0	chr7:55249071
EGFR	p.G719S	c.2155G>A	18	COSM6252	3.0	chr7:55241707
BRAF	p.V600E	c.1799T>A	15	COSM476	Endogenous mutations	chr7:140453136
KRAS	p.G12D	c.35G>A	2	COSM521	3.0	chr12:25398284
KRAS	p.Q61H	c.183A>C	3	COSM554	Endogenous mutations	chr12:25380275
PIK3CA	p.E545K	c.1633G>A	10	COSM763	3.0	chr3:178936091
PIK3CA	p.H1047R	c.3140A>G	20	COSM775	Endogenous mutations	chr3:178952085
EML4-ALK	E13-A20	DNA Translocation	N/A	COSF408	3.0	N/A
CCDC6-RET	E1-E12	DNA Translocation	N/A	COSF1271	3.0	N/A
ERBB2	CNV	Amplication	N/A	N/A	CN=4	N/A

Product application

1.Nucleic Acid Quantification and Quality Control

2.Gene Copy Number Variation Analysis

3.Genotyping and Mutation Detection:Used for the development and validation of various genotyping assays.

*Positive control: In assays such as ARMS-PCR, Sanger sequencing, and NGS, these standards provide clear comparisons of wild-type and mutant variants (e.g., EGFR L858R, KRAS G12D).

*Determining the limit of detection: By mixing mutant and wild-type standards at a specific ratio, the sensitivity of the assay can be determined. For example, determining whether a low-frequency mutation of 1% can be detected, which is crucial for tumor liquid biopsies.

*Allele Quantification: Used to accurately quantify the ratios of different alleles when analyzing methylation, mosaicism, and other conditions.

4.Next-Generation Sequencing

NGS processes are highly complex, and gDNA standards are central to quality control throughout the entire process.

*Library Construction Quality Control: As the starting material, gDNA standards monitor the efficiency and quality of library construction.

*Inter-Laboratory Comparison: Using the same set of standards allows for comparability of NGS data across different laboratories and platforms.

Related Products List

Name	Catalog No.	Details
Panel-Ref® gDNA Cocktail II Reference Standard	CBP90009	Panel-Ref® gDNA Cocktail II Reference Standard
Panel-Ref® gDNA Cocktail III Reference Standard	CBP90010	Panel-Ref® gDNA Cocktail III Reference Standard
Panel-Ref® gDNA Cocktail IV Reference Standard	CBP90011	Panel-Ref® gDNA Cocktail IV Reference Standard
Panel-Ref® gDNA Cocktail VI Reference Standard	CBP90015	Panel-Ref® gDNA Cocktail VI Reference Standard
Panel-Ref® gDNA Cocktail Ⅶ Reference Standard	CBP90016	Panel-Ref® gDNA Cocktail Ⅶ Reference Standard
Panel-Ref® gDNA Cocktail VIII Reference Standard	CBP90020	Panel-Ref® gDNA Cocktail VIII Reference Standard
Panel-Ref® gDNA Cocktail I Reference Standard	CBP90049	Panel-Ref® gDNA Cocktail I Reference Standard