Female Genomic DNA STR Std 1ug

Product Overview

The Female Genomic DNA STR Std 1ug is a specialized reference material designed for validating short tandem repeat (STR) profiling assays with a focus on gender-specific markers. This standard provides high-quality genomic DNA from a genetically characterized female donor, offering a certified control for verifying the accuracy of STR-based identity testing, particularly for gender determination and X-chromosome STR analysis. With comprehensive coverage of core STR loci and validated amelogenin markers, it is an essential tool for forensic laboratories, cell banks, and research institutions requiring reliable gender-specific controls in their authentication workflows .

Product Features

Gender-Specific Marker Validation

The standard contains a validated female genotype with homozygous X-chromosome markers and absent Y-chromosome sequences, providing a definitive control for amelogenin (AMEL) locus analysis—the primary marker for gender identification in STR profiling .

Complete Core Locus Coverage

In addition to gender markers, the standard includes verified alleles for all 13 core autosomal STR loci recommended by ANSI/ATCC standards, ensuring comprehensive validation of STR profiling workflows. Each allele’s size and intensity are calibrated for optimal performance across platforms .

High-Quality DNA Matrix

Purified to >95% genomic integrity, the DNA exhibits minimal degradation and PCR inhibition, with a concentration of 30 ng/μL in TE buffer (pH 8.0). This ensures consistent amplification efficiency and reliable peak patterns in capillary electrophoresis .

Usage

Gender Marker Validation

Incorporate the standard into validation runs to:

• Verify accurate detection of AMEL-X alleles

• Confirm absence of Y-chromosome contamination

• Establish threshold settings for gender determination

• Validate software algorithms for gender calling

STR Protocol Optimization

Use the standard to optimize STR profiling parameters:

• Determine optimal DNA input (typically 0.5-2 ng per reaction)

• Adjust PCR cycle parameters for balanced amplification

• Calibrate fragment analysis settings

• Validate new primer sets or reagent lots

Storage and Handling

Store lyophilized DNA at room temperature for up to 24 months. Once reconstituted in molecular biology-grade water, store aliquots at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles to preserve DNA integrity.

FAQ

How is the female genotype verified?

The genotype is confirmed through multiple independent analyses, including STR profiling, karyotyping, and X-chromosome inactivation studies. The certificate of analysis provides detailed allele calls for all loci.

Is this standard suitable for forensic DNA testing validation?

Yes, the standard meets forensic quality requirements, including low degradation and consistent allele representation, making it ideal for validating forensic STR kits and procedures .

Can it be used to validate X-chromosome STR panels?

Absolutely. The standard’s well-characterized X-chromosome STR loci enable validation of specialized panels used in kinship analysis and complex relationship testing.

What is the minimum number of loci verified in each lot?

Each lot undergoes comprehensive testing across 16 loci: the 13 core autosomal STRs, amelogenin, and 2 additional X-chromosome markers, ensuring robust validation of gender-specific and autosomal STR profiles .