Female X-STR Reference Standard for Cell ID

Product Overview

The Female X-STR Reference Standard for Cell ID is a premium certified reference material tailored for authenticating female-derived cell lines through short tandem repeat (STR) profiling. As cell line misidentification and cross-contamination plague 18-36% of scientific research, this standard provides a reliable benchmark for verifying cell line identity, ensuring research reproducibility and data integrity. It targets highly polymorphic X-STR loci, offering gender-specific discrimination ideal for female cell line validation. Manufactured in compliance with ISO9001 and ISO13485, this reference standard delivers consistent, traceable results for academic, pharmaceutical, and forensic applications.

Core Characteristics

Target: Polymorphic X-STR loci specific to female cell lines

Type: Lyophilized DNA reference material (100 μL reconstituted volume)

Quality grade: Research and clinical use (RUO/IVD compatible)

Compliance: Meets NIH, FDA, and journal publication requirements

Product Benefits

Superior Discrimination for Female Cell Lines

Unlike autosomal STR standards, this product focuses on X-chromosome-specific STR loci, providing enhanced discrimination power for female cell line authentication. It resolves subtle genetic differences that may be missed by generic STR panels.

Mitigates Research Risks

By enabling accurate cell line identification, the standard helps avoid the $50 billion annual cost of invalid research caused by misidentified cell lines. It ensures compliance with journal publication requirements and grant funding mandates from agencies like the NIH.

Seamless Compatibility with Industry Workflows

Optimized for use with leading STR analysis platforms (including GlobalFiler and Yfiler Plus kits), it integrates seamlessly into existing laboratory protocols. It works with both manual and automated sequencing systems, reducing workflow disruptions.

Rigorous Quality Assurance

Each batch undergoes extensive testing to ensure allele call accuracy, peak height consistency, and absence of cross-contamination. It delivers reproducible results across multiple experiments and laboratory settings.

Usage

Application Areas

Academic research: Validate female cell lines for basic science and translational studies

Biopharmaceuticals: Ensure consistency in cell-based drug development and production

Forensic science: Authenticate female-derived biological samples for STR profiling

Cell banks: Quality control for cell line preservation and distribution

Recommended Operation Steps

Reconstitute the lyophilized standard with 100 μL DNase-free water and incubate at room temperature for 15 minutes.

Amplify X-STR loci using your preferred STR amplification kit (follow manufacturer's protocol).

Analyze amplified products via capillary electrophoresis or sequencing.

Compare resulting profiles with the reference alleles provided in the COA.

Cross-verify with ICLAC or ATCC STR databases for cell line matching.

Storage and Handling

Long-term storage: 2-8°C for up to 36 months (unopened)

Short-term storage: aliquot and freeze at -20°C for 3-5 years

Transportation: Ship at ≤25°C with maximum transit time of 96 hours

Avoid exposure to direct sunlight and repeated freeze-thaw cycles

Why Choose Us

Industry-Leading Compliance

Our standard meets the strictest authentication requirements from the International Cell Line Authentication Committee (ICLAC), FDA, and NIH. It supports compliance with 21 CFR Part 11 for electronic records and signatures.

Comprehensive Locus Coverage

We include highly informative X-STR loci selected for their polymorphism and stability in female cell lines. The panel covers both mini-STRs (<220 bp) and standard STRs for optimal performance with degraded samples.

Proven Reproducibility

Tested across 50+ laboratory sites worldwide, the standard demonstrates <1% variation in allele calling. It is batch-certified to ensure consistent performance for long-term research projects.

Expert Technical Support

Our team of cell biology and STR analysis specialists provides personalized assistance, including protocol optimization and data interpretation. We also offer custom locus panels for specialized research needs.

FAQ

Can this standard be used for human and animal cell lines?

It is specifically designed for human female cell line authentication. For animal cell lines, we offer species-specific X-STR reference standards—contact our team for details.

How does this product differ from autosomal STR standards?

Autosomal STR standards target loci on non-sex chromosomes, while this product focuses on X-chromosome STRs. This provides gender-specific authentication, critical for distinguishing female cell lines from male or mixed populations.

Is the product suitable for high-throughput screening?

Yes. It is optimized for high-throughput workflows, with consistent performance in 96-well and 384-well plate formats. It works with automated liquid handling systems and high-throughput sequencers.

What documentation is included with the product?

Each order includes a detailed Certificate of Analysis (COA) with allele reference data, purity specifications, batch information, and expiration date. Compliance documentation for regulatory submissions is available upon request.