1ug Genomic DNA STR Std-2 Cell Auth

Product Overview

The 1ug Genomic DNA STR Std-2 Cell Auth is a certified reference material designed specifically for validating short tandem repeat (STR) profiling workflows in cell line authentication. This standard contains high-quality genomic DNA from two well-characterized human cell lines, providing a validated control for verifying the accuracy and precision of STR-based identity testing. With comprehensive coverage of the 13 core STR loci recommended by ANSI/ATCC ASN-0002-2022, it enables research laboratories and biopharmaceutical companies to ensure the integrity of their cell lines, preventing cross-contamination and misidentification that can compromise research results .

Product Features

Comprehensive STR Locus Coverage

The standard includes verified alleles for all 13 core STR loci (D5S818, D13S317, D7S820, D16S539, vWA, TH01, TPOX, CSF1PO, D8S1179, D3S1358, D18S51, D21S11, and FGA) plus amelogenin for gender identification. Each locus is quantified to ensure optimal peak heights and balanced amplification .

Defined Allele Frequencies

Allele sizes are calibrated against international reference standards, ensuring accurate sizing across different capillary electrophoresis platforms. The standard exhibits a discrimination power exceeding 5.02 x 10¹⁶ for unrelated individuals, meeting the highest authentication standards .

Quality-Controlled DNA

The gDNA is purified to >95% purity with A260/A280 ratios between 1.8-2.0, ensuring minimal PCR inhibition. Each lot undergoes rigorous testing for genomic integrity by agarose gel electrophoresis, showing distinct high-molecular-weight bands .

Usage

STR Protocol Validation

Use the standard to validate STR profiling protocols by:

• Verifying allele call accuracy across all 13 loci

• Optimizing PCR cycling conditions and primer concentrations

• Establishing intra-run and inter-run precision metrics

• Calibrating capillary electrophoresis instruments

Positive Control in Authentication Testing

Include the standard in every STR run as a positive control to:

• Monitor reagent performance

• Confirm proper functioning of capillary electrophoresis systems

• Provide a reference for allele sizing and interpretation

• Ensure compliance with authentication guidelines

Preparation Instructions

Reconstitute the 1 μg lyophilized DNA in 50-100 μL molecular biology-grade water or TE buffer (pH 8.0) to achieve a working concentration of 10-20 ng/μL. Allow to rehydrate at room temperature for 30 minutes with gentle mixing before use.

FAQ

What cell lines are included in this standard?

The standard contains DNA from two extensively characterized human cell lines with distinct STR profiles, selected to provide coverage of common and rare alleles across the 13 core loci. Exact cell line identities are provided in the certificate of analysis.

How does this standard support regulatory compliance?

By providing a traceable reference for STR authentication, the standard helps laboratories comply with FDA, EMA, and NIH requirements for cell line authentication in research and bioproduction .

Can it be used with automated STR analysis software?

Yes, the standard is compatible with all major STR analysis software packages. Its well-defined allele patterns facilitate software validation and ensure accurate allele calling algorithms .

What is the stability of reconstituted DNA?

Reconstituted DNA stored at 4°C remains stable for up to 30 days. For longer storage, aliquot and store at -20°C for up to 12 months, avoiding repeated freeze-thaw cycles.