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CBP20191D
CBP20191D
| Availability: | |
|---|---|
Product Overview
This product is a rigorously designed and validated DNA molecular standard. Its core component is a DNA fragment containing the specific translocation between the KMT2A gene on chromosome 11q23 and the MLLT3 gene on chromosome 9p22. The translocation breakpoint has been confirmed by precise Sanger sequencing, providing users with an authentic positive reference with clear sequence information.
The product breakpoint is constructed based on the authoritative hg19 genome build and validated by Sanger sequencing, ensuring molecular-level consistency with the genuine KMT2A-MLLT3 fusion gene found in clinical samples.
Excellent Stability: Provided in a stable Tris-EDTA buffer, it can be stored for up to 3 years at 2-8°C, preventing damage to DNA integrity from repeated freeze-thaw cycles and facilitating daily use.
Definitive Qualitative Reference: As a positive control, this product is primarily used for qualitative analysis, helping users clearly distinguish between positive and negative results. It is an ideal quality control material for molecular detection techniques such as PCR, qPCR, digital PCR, and Sanger sequencing.
Ready-to-Use Design:
Supplied in a convenient 1μg unit size, users can dilute it directly according to experimental needs, saving time on complex preparation procedures and improving lab efficiency.
Stringent Quality Control: Each batch is accompanied by a downloadable Certificate of Analysis (COA), which includes detailed DNA concentration, purity (A260/A280 ratio), and DNA electrophoresis results, guaranteeing high quality and batch-to-batch consistency.
Enhanced Detection Reliability: When establishing new detection methods or performing routine quality control, using this standard allows for effective monitoring of the entire experimental process—from nucleic acid extraction to final detection—ensuring accuracy and stability and helping to identify false negatives promptly.
Facilitation of Laboratory Standardization: The use of the same standard across different laboratories and platforms aids in the comparison and standardization of results, promoting reliable integration and exchange of data in multi-center studies.
Cost and Time Efficiency: Eliminates the need to culture cell lines or source precious clinical samples to prepare positive controls, significantly saving upfront time and material costs, allowing researchers to focus on core scientific inquiry.
Accelerated Assay Development and Optimization: Serves as a critical tool for validating the sensitivity and specificity of PCR primers, probes, or NGS panels targeting the KMT2A-MLLT3 fusion gene, significantly speeding up the R&D process.
Reduced Experimental Risk:Clear sequence information and stable quality avoid experimental failures and data bias caused by the use of positive controls of unknown origin or poor quality.
This KMT2A-MLLT3 Translocation Standard is intended for research use only. Specific applications include, but are not limited to:
Molecular Diagnostic Kit Development: Serves as a core positive control and calibrator in the development of IVD or LDT kits.
Internal Laboratory Quality Control: Acts as a daily positive control for leukemia genetic testing, ensuring the accuracy of each run.
New Technology Verification: Used to validate the detection performance of new, high-throughput, or high-sensitivity technologies such as Next-Generation Sequencing (NGS) and digital PCR.
Education and Training: Serves as a standard teaching material in molecular biology and clinical laboratory science courses to help students understand the principles and operations of translocation detection.
Drug Development and Efficacy Monitoring: Used in targeted drug development to assess the effect of compounds on cell models harboring the KMT2A-MLLT3 fusion gene.
Expertise and Focus:We specialize in providing high-quality molecular biology standards and reference materials, with an unwavering commitment to product quality.
Transparency: We provide detailed breakpoint information and publicly accessible COA certificates, giving you complete confidence in the product you are using and bolstering the credibility of your data.
Superior Stability: Our optimized formulation and storage conditions ensure consistent product performance throughout its shelf life, safeguarding the continuity of your long-term research projects.
Comprehensive Customer Support: Our team of technical specialists is ready to provide robust support for product application and related experimental design.
Exceptional Value: We are committed to providing the global research community with the highest quality products at reasonable prices, driving progress in life sciences.
This product is primarily designed as a qualitative standard. It is suitable for determining whether a detection method can effectively identify the KMT2A-MLLT3 fusion gene. While it can be used to create standard curves for semi-quantitative or quantitative assays via serial dilution, the absolute quantitative value would be for reference only. For precise quantification, a standard certified for absolute quantification is recommended.
The product should always be stored at 2-8°C. To avoid repeated freeze-thaw cycles, it is recommended to make aliquots upon first use. Always handle on ice using sterile, DNase-free labware.
You can find the link to "Download COA" on the product page. The COA provides the specific concentration, purity values, and quality control electrophoresis data for that specific batch, serving as essential documentation for product verification.
This standard is widely compatible with various molecular detection technologies, including but not limited to: Conventional PCR, Quantitative Real-Time PCR (qPCR), Sanger Sequencing, and Digital PCR (dPCR). When used with NGS, it can serve as a positive control post-library preparation to verify library quality and capture efficiency.
Why is the standard designed based on the hg19 build instead of a newer version?
The hg19 build remains widely used and referenced in many clinical assays, published studies, and commercial bioinformatics software, ensuring excellent compatibility. Providing clear breakpoint coordinates based on hg19 greatly facilitates primer design, probe matching, and result comparison for our users.
Product Overview
This product is a rigorously designed and validated DNA molecular standard. Its core component is a DNA fragment containing the specific translocation between the KMT2A gene on chromosome 11q23 and the MLLT3 gene on chromosome 9p22. The translocation breakpoint has been confirmed by precise Sanger sequencing, providing users with an authentic positive reference with clear sequence information.
The product breakpoint is constructed based on the authoritative hg19 genome build and validated by Sanger sequencing, ensuring molecular-level consistency with the genuine KMT2A-MLLT3 fusion gene found in clinical samples.
Excellent Stability: Provided in a stable Tris-EDTA buffer, it can be stored for up to 3 years at 2-8°C, preventing damage to DNA integrity from repeated freeze-thaw cycles and facilitating daily use.
Definitive Qualitative Reference: As a positive control, this product is primarily used for qualitative analysis, helping users clearly distinguish between positive and negative results. It is an ideal quality control material for molecular detection techniques such as PCR, qPCR, digital PCR, and Sanger sequencing.
Ready-to-Use Design:
Supplied in a convenient 1μg unit size, users can dilute it directly according to experimental needs, saving time on complex preparation procedures and improving lab efficiency.
Stringent Quality Control: Each batch is accompanied by a downloadable Certificate of Analysis (COA), which includes detailed DNA concentration, purity (A260/A280 ratio), and DNA electrophoresis results, guaranteeing high quality and batch-to-batch consistency.
Enhanced Detection Reliability: When establishing new detection methods or performing routine quality control, using this standard allows for effective monitoring of the entire experimental process—from nucleic acid extraction to final detection—ensuring accuracy and stability and helping to identify false negatives promptly.
Facilitation of Laboratory Standardization: The use of the same standard across different laboratories and platforms aids in the comparison and standardization of results, promoting reliable integration and exchange of data in multi-center studies.
Cost and Time Efficiency: Eliminates the need to culture cell lines or source precious clinical samples to prepare positive controls, significantly saving upfront time and material costs, allowing researchers to focus on core scientific inquiry.
Accelerated Assay Development and Optimization: Serves as a critical tool for validating the sensitivity and specificity of PCR primers, probes, or NGS panels targeting the KMT2A-MLLT3 fusion gene, significantly speeding up the R&D process.
Reduced Experimental Risk:Clear sequence information and stable quality avoid experimental failures and data bias caused by the use of positive controls of unknown origin or poor quality.
This KMT2A-MLLT3 Translocation Standard is intended for research use only. Specific applications include, but are not limited to:
Molecular Diagnostic Kit Development: Serves as a core positive control and calibrator in the development of IVD or LDT kits.
Internal Laboratory Quality Control: Acts as a daily positive control for leukemia genetic testing, ensuring the accuracy of each run.
New Technology Verification: Used to validate the detection performance of new, high-throughput, or high-sensitivity technologies such as Next-Generation Sequencing (NGS) and digital PCR.
Education and Training: Serves as a standard teaching material in molecular biology and clinical laboratory science courses to help students understand the principles and operations of translocation detection.
Drug Development and Efficacy Monitoring: Used in targeted drug development to assess the effect of compounds on cell models harboring the KMT2A-MLLT3 fusion gene.
Expertise and Focus:We specialize in providing high-quality molecular biology standards and reference materials, with an unwavering commitment to product quality.
Transparency: We provide detailed breakpoint information and publicly accessible COA certificates, giving you complete confidence in the product you are using and bolstering the credibility of your data.
Superior Stability: Our optimized formulation and storage conditions ensure consistent product performance throughout its shelf life, safeguarding the continuity of your long-term research projects.
Comprehensive Customer Support: Our team of technical specialists is ready to provide robust support for product application and related experimental design.
Exceptional Value: We are committed to providing the global research community with the highest quality products at reasonable prices, driving progress in life sciences.
This product is primarily designed as a qualitative standard. It is suitable for determining whether a detection method can effectively identify the KMT2A-MLLT3 fusion gene. While it can be used to create standard curves for semi-quantitative or quantitative assays via serial dilution, the absolute quantitative value would be for reference only. For precise quantification, a standard certified for absolute quantification is recommended.
The product should always be stored at 2-8°C. To avoid repeated freeze-thaw cycles, it is recommended to make aliquots upon first use. Always handle on ice using sterile, DNase-free labware.
You can find the link to "Download COA" on the product page. The COA provides the specific concentration, purity values, and quality control electrophoresis data for that specific batch, serving as essential documentation for product verification.
This standard is widely compatible with various molecular detection technologies, including but not limited to: Conventional PCR, Quantitative Real-Time PCR (qPCR), Sanger Sequencing, and Digital PCR (dPCR). When used with NGS, it can serve as a positive control post-library preparation to verify library quality and capture efficiency.
Why is the standard designed based on the hg19 build instead of a newer version?
The hg19 build remains widely used and referenced in many clinical assays, published studies, and commercial bioinformatics software, ensuring excellent compatibility. Providing clear breakpoint coordinates based on hg19 greatly facilitates primer design, probe matching, and result comparison for our users.
General Information
Product Information | KMT2A-MLLT3 Translocation |
| Catalog No. | CBP20019D |
Format | DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing | Download for COA |
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
General Information
Product Information | KMT2A-MLLT3 Translocation |
| Catalog No. | CBP20019D |
Format | DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing | Download for COA |
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
Detailed Data
Product Information | KMT2A-MLLT3 Translocation |
Left Gene | KMT2A |
Left Breakpoint | Chr11:1183****1(hg19) |
Right Gene | MLLT3 |
Right Breakpoint | Chr9:204****1(hg19) |
Allelic Frequency | N/A |
Buffer | Tris-EDTA |
Detailed Data
Product Information | KMT2A-MLLT3 Translocation |
Left Gene | KMT2A |
Left Breakpoint | Chr11:1183****1(hg19) |
Right Gene | MLLT3 |
Right Breakpoint | Chr9:204****1(hg19) |
Allelic Frequency | N/A |
Buffer | Tris-EDTA |
Related product list
Name | Catalog No |
KMT2A-AFF1 Translocation | CBP20193D |
KMT2A-PHLDB1 Translocation | CBP20201D |
Related product list
Name | Catalog No |
KMT2A-AFF1 Translocation | CBP20193D |
KMT2A-PHLDB1 Translocation | CBP20201D |
