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Package-Ref™ Lung Cancer SHOX2/PTGER4/RASSF1A for Methylation control

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  • CBPE0206

  • CBPE0206

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Product Description

This package contains multiple detection samples for Liver Cancer, We use simulated clinical cell lines to prepare them. If you are testing SHOX2/PTGER4/RASSF1A, you can choose this product.


Epigenetic alterations, particularly aberrant hypermethylation of DNA promoter regions, are a key mechanism in the development and progression of lung cancer. They can silence related tumor suppressor genes and promote tumor proliferation.


SHOX2 (Short Stature Homeobox 2): This gene's function is related to development. In lung cancer, its promoter region undergoes frequent and specific hypermethylation. This aberrant methylation is a highly reliable molecular marker for lung cancer, effectively detecting it in various lung cancer tissues and patient fluids (such as blood, sputum, and bronchoalveolar lavage fluid) with extremely high sensitivity.


PTGER4 (Prostaglandin E Receptor 4): Similarly, its promoter region exhibits significant hypermethylation in lung cancer cells. This epigenetic alteration is associated with gene transcriptional repression and is considered an early event in lung cancer development, making it an excellent diagnostic marker.


RASSF1A (Ras association domain family member 1A): This is a classic tumor suppressor gene. Hypermethylation of its promoter directly silences the gene, causing it to lose its functions of inhibiting cell growth and promoting apoptosis, thereby driving tumor progression. RASSF1A methylation is one of the most common epigenetic abnormalities in human cancer and is highly detected in lung cancer.


In lung cancer methylation testing based on liquid biopsies (such as blood and bronchoalveolar lavage fluid), they are used as intrinsic quality control of the detection system.


General information

Name

Package-Ref™ Lung Cancer SHOX2/PTGER4/RASSF1A for Methylation control

Cat. No.

CBPE0206

Format

Genomic DNA

Size

1ug

Buffer

Tris-EDTA

Intended Use

Research Use Only

Concentration

Download for COA

Purity

Download for COA

DNA electrophoresis

Download for COA

Storage Conditions

2~8℃

Expiry

36 months from the date of manufacture

Remark

The data in the instruction manual is the data of a single test, and the specific data of each batch is subject to delivery.

 

Technical Data

Classification                

Cat No.                

Name                

Description                

Region                

Methylation level                

quality control standard                

CBPE0206-1                

Lung Cancer-SHOX2,PTGER4,RASSF1A Positive methylation                

SHOX2 Methylation Positive control                

chr3:157821337-157821430(hg19)                

97.43%                

PTGER4 Methylation Positive control                

chr5:40681482-40681593(hg19)                

99.76%                

PTGER4 Methylation Positive control                

chr5:40681898-40682110(hg19)                

96.26%                

RASSF1A Methylation Positive control                

chr3:50378322-50378233(hg19)                

99.33%                

CBPE0206-2                

Lung Cancer-SHOX2,PTGER4,RASSF1A Negative methylation                

SHOX2 Methylation Negative control                

chr3:157821337-157821430(hg19)                

0.00%                

PTGER4 Methylation Negative control                

chr5:40681482-40681593(hg19)                

0.19%                

PTGER4 Methylation Negative control                

chr5:40681898-40682110(hg19)                

0.05%                

RASSF1A Methylation Negative control                

chr3:50378322-50378233(hg19)                

0.02%                

CBPE0206-3                

Lung Cancer-PTGER4 High Positive methylation reference，SHOX2 Negative methylation,RASSF1A Negative methylation                

SHOX2 Methylation Negative control                

chr3:157821337-157821430(hg19)                

0.12%                

PTGER4 Methylation Positive control                

chr5:40681482-40681593(hg19)                

79.25%                

PTGER4  Methylation Positive control                

chr5:40681898-40682110(hg19)                

84.78%                

RASSF1A Methylation Negative control                

chr3:50378322-50378233(hg19)                

0.00%                

CBPE0206-4                

Lung Cancer-SHOX2,PTGER4 Negative methylation，RASSF1A Positive methylation                

SHOX2 Methylation Negative control                

chr3:157821337-157821430(hg19)                

0.12%                

PTGER4 Methylation Negative control                

chr5:40681482-40681593(hg19)                

0.54%                

PTGER4  Methylation Negative control                

chr5:40681898-40682110(hg19)                

0.00%                

RASSF1A Methylation Positive control                

chr3:50378322-50378233(hg19)                

81.66%                

CBPE0206-5                

Lung Cancer-RASSF1A,PTGER4 Negative methylation，SHOX2 Positive methylation                

SHOX2 Methylation Positive control                

chr3:157821337-157821430(hg19)                

50.32%                

PTGER4 Methylation Negative control                

chr5:40681482-40681593(hg19)                

0.78%                

PTGER4 Methylation Negative control                

chr5:40681898-40682110(hg19)                

0.40%                

RASSF1A Methylation Negative control                

chr3:50378322-50378233(hg19)                

0.00%                








Classification                


Cat No.                

Name                

Description                

Region                

Methylation level                

Single Positive SHOX2-reference standard                

Single Positive SHOX2 segment-High Positive methylation reference-1                

CBPE0206-6                

SHOX2 High Positive methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Reference Standard with 50.00%                

chr3:157821337-157821430 (hg19)                

50.32%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.78%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.40%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.00%                

Single Positive SHOX2 segment-High Positive methylation reference-2                

CBPE0206-7                

SHOX2 High Positive methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Reference Standard with 10.00%                

chr3:157821337-157821430 (hg19)                

9.44%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.00%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.00%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.00%                

Single Positive SHOX2 segment-Low Positive reference standard                

CBPE0206-8                

SHOX2 Low Positive methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Reference Standard with 3.00%                

chr3:157821337-157821430 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

N/A                

Single Positive SHOX2 segment- LOD reference                

CBPE0206-9                

SHOX2 LOD methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Reference Standard with 1.00%                

chr3:157821337-157821430 (hg19)                

1.57%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.30%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.79%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.00%                

Single Positive SHOX2 segment-Negative reference                

CBPE0206-10                

SHOX2 Negative methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                

Single Positive SHOX2 segment-Precision reference                

CBPE0206-11                

SHOX2 Low Positive methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Reference Standard with 3.00%                

chr3:157821337-157821430 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

N/A                

CBPE0206-12                

SHOX2 Negative methylation reference，RASSF1A,PTGER4 Negative methylation                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                








Classification                


Cat No.                

Name                

Description                

Region                

Methylation level                

Single Positive PTGER4-reference standard                

Single Positive PTGER4 segment-High Positive methylation reference-1                

CBPE0206-13                

PTGER4 High Positive methylation reference，SHOX2 Negative methylation，RASSF1A Negative methylation                

PTGER4 Methylation Reference Standard with 75.00%                

chr5:40681482-40681593 (hg19)                

79.25%                

PTGER4 Methylation Reference Standard with 80.00%                

chr5:40681898-40682110 (hg19)                

84.78%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.00%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.12%                

Single Positive PTGER4 segment-High Positive methylation reference-2                

CBPE0206-14                

PTGER4 High Positive methylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Reference Standard with 10.00%                

chr5:40681482-40681593 (hg19)                

11.17%                

PTGER4 Methylation Reference Standard with 10.00%                

chr5:40681898-40682110 (hg19)                

11.33%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.21%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.08%                

Single Positive PTGER4 segment-Low Positive reference standard                

CBPE0206-15                

PTGER4 Low Positive methylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Reference Standard with 3.00%                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Reference Standard with 3.00%                

chr5:40681898-40682110 (hg19)                

N/A                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

Single Positive PTGER4 segment- LOD reference                

CBPE0206-16                

PTGER4 LOD methylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Reference Standard with 1.00%                

chr5:40681482-40681593 (hg19)                

1.30%                

PTGER4 Methylation Reference Standard with 1.00%                

chr5:40681898-40682110 (hg19)                

2.35%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.00%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.18%                

Single Positive PTGER4 segment-Negative reference                

CBPE0206-17                

PTGER4 Negative methylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                

Single Positive PTGER4 segment-Precision reference                

CBPE0206-18                

PTGER4 Low Positivemethylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Reference Standard with 3.00%                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

CBPE0206-19                

PTGER4Negative methylation reference，RASSF1A,SHOX2 Negative methylation                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                








Classification                


Cat No.                

Name                

Description                

Region                

Methylation level                

Single Positive RASSF1A-reference standard                

Single Positive RASSF1A segment-High Positive methylation reference-1                

CBPE0206-20                

RASSF1A High Positive methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Reference Standard with 80.00%                

chr3:50378322-50378233 (hg19)                

81.66%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.54%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.00%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.12%                

Single Positive RASSF1A segment-High Positive methylation reference-2                

CBPE0206-21                

RASSF1A High Positive methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Reference Standard with 10.00%                

chr3:50378322-50378233 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

Single Positive RASSF1A segment-Low Positive reference standard                

CBPE0206-22                

RASSF1A Low Positive methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Reference Standard with 3.00%                

chr3:50378322-50378233 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

Single Positive RASSF1A segment- LOD reference                

CBPE0206-23                

RASSF1A LOD methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Reference Standard with 1.00%                

chr3:50378322-50378233 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

Single Positive RASSF1A segment-Negative reference                

CBPE0206-24                

RASSF1A Negative methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                

Single Positive RASSF1A segment-Precision reference                

CBPE0206-25                

RASSF1A Low Positive methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Reference Standard with 3.00%                

chr3:50378322-50378233 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

N/A                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

N/A                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

N/A                

CBPE0206-26                

RASSF1A4Negative methylation reference，PTGER4,SHOX2 Negative methylation                

RASSF1A Methylation Negative Reference Standard                

chr3:50378322-50378233 (hg19)                

0.02%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681482-40681593 (hg19)                

0.19%                

PTGER4 Methylation Negative Reference Standard                

chr5:40681898-40682110 (hg19)                

0.05%                

SHOX2 Methylation Negative Reference Standard                

chr3:157821337-157821430 (hg19)                

0.00%                

Remark                

1.Quality control products are also called accuracy standards and are used for qualitative judgment;                

2. Reference products are used for qualitative and quantitative judgments. The judgment of strong positive, weak positive, detection limit, precision and specificity depends on the specific project. The judgment of this product is only an internal standard;                

3. Quality control products are used for performance evaluation, factory inspection, and daily detection and judgment of test kits. Reference products are used for performance evaluation and factory inspection;                

4. The above products only show part of the results, and the degree of methylation can be customized.                


Application Scenario

① Internal Control of the Testing Process: In a methylation assay for a clinical sample, failures in any of the steps—from DNA extraction and sulfite conversion (a key step that converts unmethylated C to U) to the final PCR amplification and detection—can result in a false-negative result. If a sample highly suspected of lung cancer (such as bronchoalveolar lavage fluid) fails to detect methylation signals for any of these three genes, this strongly suggests a possible protocol issue (such as DNA degradation, low conversion efficiency, or PCR inhibition), indicating that the test results are unreliable and require repeat testing. This effectively avoids missed diagnoses due to technical errors.


②Complementary and Synergistic Biomarker Panels: Combining these three genes into a single test panel can cover a wider range of lung cancer patients and improve overall test sensitivity. The methylation profiles of cancer cells may vary slightly between patients. Simultaneously testing multiple markers maximizes the capture of positive signals and ensures test reliability.


③Ensuring Reliable Result Interpretation: As stable positive internal controls, they help confirm that the detection system itself is functioning properly, enabling researchers to confidently interpret the methylation status of other target genes (whether positive or negative), ultimately producing an accurate and reliable clinical test report.


In summary, SHOX2, PTGER4, and RASSF1A, due to their high frequency and specific methylation in lung cancer, are used in combination as "quality controls" in lung cancer methylation testing. Their primary function is to monitor the quality of the entire experimental process, ensure the accuracy and reliability of test results, and avoid false negatives. They play a crucial quality control role in the non-invasive early diagnosis and efficacy monitoring of lung cancer.


General information

Name

Package-Ref™ Lung Cancer SHOX2/PTGER4/RASSF1A for Methylation control

Cat. No.

CBPE0206

Format

Genomic DNA

Size

1ug

Buffer

Tris-EDTA

Intended Use

Research Use Only

Concentration

Download for COA

Purity

Download for COA

DNA electrophoresis

Download for COA

Storage Conditions

2~8℃

Expiry

36 months from the date of manufacture

Remark

The data in the instruction manual is the data of a single test, and the specific data of each batch is subject to delivery.



Technical Data

Classification

Cat No.

Name

Description

Region

Methylation level

quality control standard

CBPE0206-1

Lung Cancer-SHOX2,PTGER4,RASSF1A Positive methylation

SHOX2 Methylation Positive control

chr3:157821337-157821430(hg19)

97.43%

PTGER4 Methylation Positive control

chr5:40681482-40681593(hg19)

99.76%

PTGER4 Methylation Positive control

chr5:40681898-40682110(hg19)

96.26%

RASSF1A Methylation Positive control

chr3:50378322-50378233(hg19)

99.33%

CBPE0206-2

Lung Cancer-SHOX2,PTGER4,RASSF1A Negative methylation

SHOX2 Methylation Negative control

chr3:157821337-157821430(hg19)

0.00%

PTGER4 Methylation Negative control

chr5:40681482-40681593(hg19)

0.19%

PTGER4 Methylation Negative control

chr5:40681898-40682110(hg19)

0.05%

RASSF1A Methylation Negative control

chr3:50378322-50378233(hg19)

0.02%

CBPE0206-3

Lung Cancer-PTGER4 High Positive methylation reference，SHOX2 Negative methylation,RASSF1A Negative methylation

SHOX2 Methylation Negative control

chr3:157821337-157821430(hg19)

0.12%

PTGER4 Methylation Positive control

chr5:40681482-40681593(hg19)

79.25%

PTGER4  Methylation Positive control

chr5:40681898-40682110(hg19)

84.78%

RASSF1A Methylation Negative control

chr3:50378322-50378233(hg19)

0.00%

CBPE0206-4

Lung Cancer-SHOX2,PTGER4 Negative methylation，RASSF1A Positive methylation

SHOX2 Methylation Negative control

chr3:157821337-157821430(hg19)

0.12%

PTGER4 Methylation Negative control

chr5:40681482-40681593(hg19)

0.54%

PTGER4  Methylation Negative control

chr5:40681898-40682110(hg19)

0.00%

RASSF1A Methylation Positive control

chr3:50378322-50378233(hg19)

81.66%

CBPE0206-5

Lung Cancer-RASSF1A,PTGER4 Negative methylation，SHOX2 Positive methylation

SHOX2 Methylation Positive control

chr3:157821337-157821430(hg19)

50.32%

PTGER4 Methylation Negative control

chr5:40681482-40681593(hg19)

0.78%

PTGER4 Methylation Negative control

chr5:40681898-40682110(hg19)

0.40%

RASSF1A Methylation Negative control

chr3:50378322-50378233(hg19)

0.00%








Classification


Cat No.

Name

Description

Region

Methylation level

Single Positive SHOX2-reference standard

Single Positive SHOX2 segment-High Positive methylation reference-1

CBPE0206-6

SHOX2 High Positive methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Reference Standard with 50.00%

chr3:157821337-157821430 (hg19)

50.32%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.78%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.40%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.00%

Single Positive SHOX2 segment-High Positive methylation reference-2

CBPE0206-7

SHOX2 High Positive methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Reference Standard with 10.00%

chr3:157821337-157821430 (hg19)

9.44%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.00%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.00%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.00%

Single Positive SHOX2 segment-Low Positive reference standard

CBPE0206-8

SHOX2 Low Positive methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Reference Standard with 3.00%

chr3:157821337-157821430 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

N/A

Single Positive SHOX2 segment- LOD reference

CBPE0206-9

SHOX2 LOD methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Reference Standard with 1.00%

chr3:157821337-157821430 (hg19)

1.57%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.30%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.79%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.00%

Single Positive SHOX2 segment-Negative reference

CBPE0206-10

SHOX2 Negative methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%

Single Positive SHOX2 segment-Precision reference

CBPE0206-11

SHOX2 Low Positive methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Reference Standard with 3.00%

chr3:157821337-157821430 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

N/A

CBPE0206-12

SHOX2 Negative methylation reference，RASSF1A,PTGER4 Negative methylation

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%








Classification


Cat No.

Name

Description

Region

Methylation level

Single Positive PTGER4-reference standard

Single Positive PTGER4 segment-High Positive methylation reference-1

CBPE0206-13

PTGER4 High Positive methylation reference，SHOX2 Negative methylation，RASSF1A Negative methylation

PTGER4 Methylation Reference Standard with 75.00%

chr5:40681482-40681593 (hg19)

79.25%

PTGER4 Methylation Reference Standard with 80.00%

chr5:40681898-40682110 (hg19)

84.78%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.00%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.12%

Single Positive PTGER4 segment-High Positive methylation reference-2

CBPE0206-14

PTGER4 High Positive methylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Reference Standard with 10.00%

chr5:40681482-40681593 (hg19)

11.17%

PTGER4 Methylation Reference Standard with 10.00%

chr5:40681898-40682110 (hg19)

11.33%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.21%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.08%

Single Positive PTGER4 segment-Low Positive reference standard

CBPE0206-15

PTGER4 Low Positive methylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Reference Standard with 3.00%

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Reference Standard with 3.00%

chr5:40681898-40682110 (hg19)

N/A

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

Single Positive PTGER4 segment- LOD reference

CBPE0206-16

PTGER4 LOD methylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Reference Standard with 1.00%

chr5:40681482-40681593 (hg19)

1.30%

PTGER4 Methylation Reference Standard with 1.00%

chr5:40681898-40682110 (hg19)

2.35%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.00%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.18%

Single Positive PTGER4 segment-Negative reference

CBPE0206-17

PTGER4 Negative methylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%

Single Positive PTGER4 segment-Precision reference

CBPE0206-18

PTGER4 Low Positivemethylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Reference Standard with 3.00%

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

CBPE0206-19

PTGER4Negative methylation reference，RASSF1A,SHOX2 Negative methylation

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%








Classification


Cat No.

Name

Description

Region

Methylation level

Single Positive RASSF1A-reference standard

Single Positive RASSF1A segment-High Positive methylation reference-1

CBPE0206-20

RASSF1A High Positive methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Reference Standard with 80.00%

chr3:50378322-50378233 (hg19)

81.66%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.54%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.00%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.12%

Single Positive RASSF1A segment-High Positive methylation reference-2

CBPE0206-21

RASSF1A High Positive methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Reference Standard with 10.00%

chr3:50378322-50378233 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

Single Positive RASSF1A segment-Low Positive reference standard

CBPE0206-22

RASSF1A Low Positive methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Reference Standard with 3.00%

chr3:50378322-50378233 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

Single Positive RASSF1A segment- LOD reference

CBPE0206-23

RASSF1A LOD methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Reference Standard with 1.00%

chr3:50378322-50378233 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

Single Positive RASSF1A segment-Negative reference

CBPE0206-24

RASSF1A Negative methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%

Single Positive RASSF1A segment-Precision reference

CBPE0206-25

RASSF1A Low Positive methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Reference Standard with 3.00%

chr3:50378322-50378233 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

N/A

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

N/A

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

N/A

CBPE0206-26

RASSF1A4Negative methylation reference，PTGER4,SHOX2 Negative methylation

RASSF1A Methylation Negative Reference Standard

chr3:50378322-50378233 (hg19)

0.02%

PTGER4 Methylation Negative Reference Standard

chr5:40681482-40681593 (hg19)

0.19%

PTGER4 Methylation Negative Reference Standard

chr5:40681898-40682110 (hg19)

0.05%

SHOX2 Methylation Negative Reference Standard

chr3:157821337-157821430 (hg19)

0.00%

Remark

1.Quality control products are also called accuracy standards and are used for qualitative judgment;

2. Reference products are used for qualitative and quantitative judgments. The judgment of strong positive, weak positive, detection limit, precision and specificity depends on the specific project. The judgment of this product is only an internal standard;

3. Quality control products are used for performance evaluation, factory inspection, and daily detection and judgment of test kits. Reference products are used for performance evaluation and factory inspection;

4. The above products only show part of the results, and the degree of methylation can be customized.


Application Scenario

① Internal Control of the Testing Process: In a methylation assay for a clinical sample, failures in any of the steps—from DNA extraction and sulfite conversion (a key step that converts unmethylated C to U) to the final PCR amplification and detection—can result in a false-negative result. If a sample highly suspected of lung cancer (such as bronchoalveolar lavage fluid) fails to detect methylation signals for any of these three genes, this strongly suggests a possible protocol issue (such as DNA degradation, low conversion efficiency, or PCR inhibition), indicating that the test results are unreliable and require repeat testing. This effectively avoids missed diagnoses due to technical errors.


②Complementary and Synergistic Biomarker Panels: Combining these three genes into a single test panel can cover a wider range of lung cancer patients and improve overall test sensitivity. The methylation profiles of cancer cells may vary slightly between patients. Simultaneously testing multiple markers maximizes the capture of positive signals and ensures test reliability.


③Ensuring Reliable Result Interpretation: As stable positive internal controls, they help confirm that the detection system itself is functioning properly, enabling researchers to confidently interpret the methylation status of other target genes (whether positive or negative), ultimately producing an accurate and reliable clinical test report.


In summary, SHOX2, PTGER4, and RASSF1A, due to their high frequency and specific methylation in lung cancer, are used in combination as "quality controls" in lung cancer methylation testing. Their primary function is to monitor the quality of the entire experimental process, ensure the accuracy and reliability of test results, and avoid false negatives. They play a crucial quality control role in the non-invasive early diagnosis and efficacy monitoring of lung cancer.



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