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Product Overview
ERBB2 (also known as HER2) is a crucial receptor tyrosine kinase belonging to the Epidermal Growth Factor Receptor (EGFR) family. It plays a key role in cell growth, differentiation, and transformation by activating the PI3K-AKT-mTOR and RAS-RAF-MEK-ERK signaling pathways (PMID: 17471238). Amplification, overexpression, and aberrant activation of ERBB2 are closely associated with the development and progression of various tumors, particularly in breast cancer, gastric cancer, and ovarian cancer (PMID: 17471238). Consequently, ERBB2 has become a vital target in cancer research and clinical diagnostics.
To meet the research needs for studying ERBB2 gene mutations, we introduce the ERBB2 Standard (c.2326_2327insTGT, p.G776>VC). This standard is designed based on transcript NM_004448.3, accurately mimicking a typical insertion mutation in the ERBB2 gene. It is suitable for various research applications such as mutation detection, assay validation, and experimental quality control. This product is intended for Research Use Only (RUO), providing researchers with a reliable and efficient experimental tool.
The ERBB2 Standard contains the c.2326_2327insTGT mutation, resulting in the amino acid change p.G776>VC. This "In-Frame Insertion" mutation type replicates a common mutation pattern of the ERBB2 gene in tumors, aiding researchers in accurately evaluating the sensitivity and specificity of detection methods.
This product is prepared in Tris-EDTA buffer, ensuring high DNA purity and stability. The accuracy and consistency of the mutation site are confirmed through rigorous DNA electrophoresis and Sanger sequencing validation
Well-Defined Genetic Background
The mutation is located at chr17:37880997_37880998 (GRCh37). Its heterozygous design more closely reflects the mutational status found in real samples, with an allelic frequency of 50%, making it compatible with most molecular detection platforms.
The standard remains stable for 36 months when stored at 2-8°C, facilitating long-term laboratory use and reducing the risk of product degradation due to improper storage conditions.
High Reliability: The ERBB2 Standard undergoes multiple rounds of quality control, including concentration measurement, purification verification, and mutation site confirmation, ensuring that each batch meets high standards. Researchers can confidently use it for the development and optimization of mutation detection assays.
Broad Applicability: This standard is suitable for various molecular biology techniques, such as PCR, Sanger sequencing, Next-Generation Sequencing (NGS), and digital PCR. It provides reliable support for both basic research and pre-clinical validation.
Simplified Workflow: Provided as ready-to-use DNA, the product eliminates the need for additional DNA extraction or purification steps, significantly saving experimental time and cost. Researchers can focus on data analysis and interpretation rather than sample preparation.
Streamlines Workflow: The ready-to-use gDNA format eliminates the need for tedious cell culture or DNA extraction steps, saving valuable research time.
Facilitates Research Progress: The ERBB2 Standard helps researchers gain deeper insights into the role of ERBB2 mutations in tumorigenesis and serves as a vital tool for developing targeted therapies against ERBB2.
The ERBB2 Standard is primarily used in the following research areas:
Serves as a positive control to help validate and optimize ERBB2 mutation detection methods, ensuring accurate and reproducible results.
Laboratory Quality Control: Used for internal quality assessment to monitor the stability and consistency of detection workflows.
Pre-Clinical Research: Models the ERBB2 mutation in cell lines or animal models to study its biological function and impact on drug sensitivity.
Education and Training: Acts as a teaching tool for training and demonstration in molecular biology and cancer genetics.
Expert Technical Support: We have years of experience in developing gene mutation standards and are committed to providing high-quality products and professional technical services to researchers..
Stringent Quality Control: Each batch undergoes multiple validation steps, including DNA electrophoresis, Sanger sequencing, and concentration measurement, ensuring product consistency and reliability.
User-Friendly Design: The product comes with detailed technical parameters and usage instructions, including DNA concentration, purification method, and storage conditions, enabling users to get started quickly.
Comprehensive Customer Service: We provide excellent technical support and after-sales service to help resolve issues encountered during experiments, ensuring smooth research progress.
The ERBB2 Standard is primarily used for the development and validation of mutation detection assays, laboratory quality control, pre-clinical research, and education/training. It helps researchers evaluate the sensitivity, specificity, and reproducibility of detection technologies.
The mutation is an In-Frame Insertion, specifically c.2326_2327insTGT, leading to the amino acid change p.G776>VC. This mutation mimics a common variant form of ERBB2 in tumors, which is significant for studying its function and targeted therapies.
The product should be stored at 2-8°C and has a shelf life of 36 months from the date of manufacture. Avoid repeated freeze-thaw cycles to maintain DNA stability and integrity.
We verify the mutation site for each batch through Sanger sequencing and DNA electrophoresis. Users can also download the provided Certificate of Analysis (COA) for detailed information.
Is this product suitable for Next-Generation Sequencing (NGS) platforms?
Yes, the ERBB2 Standard is suitable for various molecular detection techniques, including NGS, Sanger sequencing, and PCR, providing a reliable positive control for mutation analysis.
What is the zygosity design of the standard?
The standard is designed as Heterozygous, with an allelic frequency of 50%, which more closely resembles the mutational status found in actual samples.
Product Information | AI-Edigene® ERBB2 p.G776>VC Reference Standard plus |
| Catalog No. | CBP10473 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® ERBB2 p.G776>VC Reference Standard plus |
DNA Change | c.2326_2327insTGT |
AA Change | p.G776>VC |
Mutation type | In_Frame_Ins |
Zygosity | Heterozygous |
Allelic Frequency | 50% |
| Transcript | NM_004448.3 |
Cosmic ID | N/A |
Chr position(GRCh37) | chr17:37880997_37880998 |
Buffer | Tris-EDTA |
Related product list
Name | Catalog No. |
AI-Edigene® ERBB2 p.A775_G776insYVMA Reference Standard Plus | CBP10416 |
AI-Edigene® ERBB2 p.V777L Reference Standard Plus | CBP10433 |
AI-Edigene® ERBB2 p.P780_Y781insGSP Reference Standard Plus | CBP10472 |
AI-Edigene® ERBB2 p.V777_G778insCG Reference Standard Plus | CBP10474 |
If you have any questions, please feel free to contact us at sales@cb-gene.com
Product Overview
ERBB2 (also known as HER2) is a crucial receptor tyrosine kinase belonging to the Epidermal Growth Factor Receptor (EGFR) family. It plays a key role in cell growth, differentiation, and transformation by activating the PI3K-AKT-mTOR and RAS-RAF-MEK-ERK signaling pathways (PMID: 17471238). Amplification, overexpression, and aberrant activation of ERBB2 are closely associated with the development and progression of various tumors, particularly in breast cancer, gastric cancer, and ovarian cancer (PMID: 17471238). Consequently, ERBB2 has become a vital target in cancer research and clinical diagnostics.
To meet the research needs for studying ERBB2 gene mutations, we introduce the ERBB2 Standard (c.2326_2327insTGT, p.G776>VC). This standard is designed based on transcript NM_004448.3, accurately mimicking a typical insertion mutation in the ERBB2 gene. It is suitable for various research applications such as mutation detection, assay validation, and experimental quality control. This product is intended for Research Use Only (RUO), providing researchers with a reliable and efficient experimental tool.
The ERBB2 Standard contains the c.2326_2327insTGT mutation, resulting in the amino acid change p.G776>VC. This "In-Frame Insertion" mutation type replicates a common mutation pattern of the ERBB2 gene in tumors, aiding researchers in accurately evaluating the sensitivity and specificity of detection methods.
This product is prepared in Tris-EDTA buffer, ensuring high DNA purity and stability. The accuracy and consistency of the mutation site are confirmed through rigorous DNA electrophoresis and Sanger sequencing validation
Well-Defined Genetic Background
The mutation is located at chr17:37880997_37880998 (GRCh37). Its heterozygous design more closely reflects the mutational status found in real samples, with an allelic frequency of 50%, making it compatible with most molecular detection platforms.
The standard remains stable for 36 months when stored at 2-8°C, facilitating long-term laboratory use and reducing the risk of product degradation due to improper storage conditions.
High Reliability: The ERBB2 Standard undergoes multiple rounds of quality control, including concentration measurement, purification verification, and mutation site confirmation, ensuring that each batch meets high standards. Researchers can confidently use it for the development and optimization of mutation detection assays.
Broad Applicability: This standard is suitable for various molecular biology techniques, such as PCR, Sanger sequencing, Next-Generation Sequencing (NGS), and digital PCR. It provides reliable support for both basic research and pre-clinical validation.
Simplified Workflow: Provided as ready-to-use DNA, the product eliminates the need for additional DNA extraction or purification steps, significantly saving experimental time and cost. Researchers can focus on data analysis and interpretation rather than sample preparation.
Streamlines Workflow: The ready-to-use gDNA format eliminates the need for tedious cell culture or DNA extraction steps, saving valuable research time.
Facilitates Research Progress: The ERBB2 Standard helps researchers gain deeper insights into the role of ERBB2 mutations in tumorigenesis and serves as a vital tool for developing targeted therapies against ERBB2.
The ERBB2 Standard is primarily used in the following research areas:
Serves as a positive control to help validate and optimize ERBB2 mutation detection methods, ensuring accurate and reproducible results.
Laboratory Quality Control: Used for internal quality assessment to monitor the stability and consistency of detection workflows.
Pre-Clinical Research: Models the ERBB2 mutation in cell lines or animal models to study its biological function and impact on drug sensitivity.
Education and Training: Acts as a teaching tool for training and demonstration in molecular biology and cancer genetics.
Expert Technical Support: We have years of experience in developing gene mutation standards and are committed to providing high-quality products and professional technical services to researchers..
Stringent Quality Control: Each batch undergoes multiple validation steps, including DNA electrophoresis, Sanger sequencing, and concentration measurement, ensuring product consistency and reliability.
User-Friendly Design: The product comes with detailed technical parameters and usage instructions, including DNA concentration, purification method, and storage conditions, enabling users to get started quickly.
Comprehensive Customer Service: We provide excellent technical support and after-sales service to help resolve issues encountered during experiments, ensuring smooth research progress.
The ERBB2 Standard is primarily used for the development and validation of mutation detection assays, laboratory quality control, pre-clinical research, and education/training. It helps researchers evaluate the sensitivity, specificity, and reproducibility of detection technologies.
The mutation is an In-Frame Insertion, specifically c.2326_2327insTGT, leading to the amino acid change p.G776>VC. This mutation mimics a common variant form of ERBB2 in tumors, which is significant for studying its function and targeted therapies.
The product should be stored at 2-8°C and has a shelf life of 36 months from the date of manufacture. Avoid repeated freeze-thaw cycles to maintain DNA stability and integrity.
We verify the mutation site for each batch through Sanger sequencing and DNA electrophoresis. Users can also download the provided Certificate of Analysis (COA) for detailed information.
Is this product suitable for Next-Generation Sequencing (NGS) platforms?
Yes, the ERBB2 Standard is suitable for various molecular detection techniques, including NGS, Sanger sequencing, and PCR, providing a reliable positive control for mutation analysis.
What is the zygosity design of the standard?
The standard is designed as Heterozygous, with an allelic frequency of 50%, which more closely resembles the mutational status found in actual samples.
Product Information | AI-Edigene® ERBB2 p.G776>VC Reference Standard plus |
| Catalog No. | CBP10473 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® ERBB2 p.G776>VC Reference Standard plus |
DNA Change | c.2326_2327insTGT |
AA Change | p.G776>VC |
Mutation type | In_Frame_Ins |
Zygosity | Heterozygous |
Allelic Frequency | 50% |
| Transcript | NM_004448.3 |
Cosmic ID | N/A |
Chr position(GRCh37) | chr17:37880997_37880998 |
Buffer | Tris-EDTA |
Related product list
Name | Catalog No. |
AI-Edigene® ERBB2 p.A775_G776insYVMA Reference Standard Plus | CBP10416 |
AI-Edigene® ERBB2 p.V777L Reference Standard Plus | CBP10433 |
AI-Edigene® ERBB2 p.P780_Y781insGSP Reference Standard Plus | CBP10472 |
AI-Edigene® ERBB2 p.V777_G778insCG Reference Standard Plus | CBP10474 |
If you have any questions, please feel free to contact us at sales@cb-gene.com
