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Product Overview
The AI-Edigene® EGFR VIII(E2-E7 Del) Fusion is a rigorously designed and quality-controlled, in vitro transcribed RNA reference material that accurately mimics the transcript of the naturally occurring EGFR vIII fusion gene in humans. This variant is formed by an in-frame deletion of exons 2 through 7 (E2-E7) of the EGFR gene, resulting in a protein lacking 267 amino acids in the extracellular domain, which leads to constitutive, ligand-independent oncogenic activation.
Technical Specifications
Format: High-purity RNA
Fusion Gene: EGFR-EGFR (E2-E7 Del)
Genomic Coordinates: Left Breakpoint chr7:55087058:+; Right Breakpoint chr7:55223523:+
Gene Background: Human EGFR gene
Buffer: RNase-free H2O, maximizing RNA integrity
Purity & Concentration: Detailed Certificate of Analysis (COA) provided for traceability
Storage Conditions: -90°C ~ -70°C (Ultra-low temperature freezer)
Shelf Life: 12 months from the date of manufacture
Intended Use: For Research Use Only. Not for use in diagnostic procedures.
With its clearly defined genomic breakpoints, this product serves as a molecular "ruler" for your experiments, forming the foundation for precise qualitative and quantitative analysis.
The sequence is strictly designed according to the known EGFR vIII (E2-E7 Del) sequence, with defined breakpoints (chr7:55087058:+ and chr7:55223523:+). It is verified by Sanger sequencing, ensuring high concordance with the variant sequence found in actual clinical samples.
The product undergoes stringent purification to remove impurities and enzyme residues. RNA electrophoresis confirms excellent integrity with no signs of degradation, allowing direct use in downstream experiments and guaranteeing reliable results.
Preserved in RNase-free water and stored at -90°C ~ -70°C, it is effectively protected from RNA degradation, ensuring consistent performance and low inter-batch variation throughout its shelf life.
Ready-to-Use Design
Upon receipt, users can simply dilute or aliquot the product as needed for their experiments, saving significant time and cost associated with in-house preparation.
Accurate Quantification
The product is supplied with precise concentration data (see COA), supporting absolute quantification analysis, such as generating standard curves for dPCR or NGS platforms.
When used as a positive control during the development of RT-PCR, ddPCR, or NGS panels, it helps optimize reaction conditions, ensuring the assay specifically identifies the EGFR vIII fusion and avoids false-negative or false-positive results.
Using the same standard across different experiment batches allows for effective monitoring of process stability and evaluation of operator performance and reagent quality, ensuring long-term comparability and reproducibility of data.
Accelerates Assay Development and Validation
For newly established detection methods, this standard acts as a "gold standard" to verify success and reliability, significantly shortening the development-to-application timeline.
Facilitates Cross-Platform Comparison
It can be used as a universal reference for comparing results and standardizing data across different technological platforms, such as qPCR, dPCR, and NGS, promoting multi-center research collaboration.
Eliminates Risk of Sample Cross-Contamination
Using a synthetic standard instead of patient-derived material completely avoids biosafety risks and the potential for cross-contamination between samples, ensuring safer and more ethically compliant operations.
Assay Development & Optimization: For developing and optimizing detection schemes for EGFR vIII using RT-qPCR, ddPCR, NanoString, or NGS.
Internal Quality Control (IQC): Serves as a positive control and quantitative standard for each assay run, monitoring the stability of the entire workflow (from RNA extraction to data analysis).
Kit Performance Evaluation: Used to evaluate the sensitivity, specificity, and limit of detection (LoD) of commercial EGFR mutation detection kits.
Laboratory Proficiency Testing & Standardization: Acts as a third-party quality control material for inter-laboratory proficiency testing and assay harmonization.
Basic Mechanism Research: In cell model studies, it can be used as a template for transfection to study the biological functions of EGFR vIII and its impact on drug responses.
1.Precision at Source, Superior Quality: We control quality from the source, ensuring accuracy at the single-base level. Multiple QC techniques, including electrophoresis and sequencing, are employed, and all key data are provided via a downloadable COA for full transparency.
2.Specialization and Deep Expertise: We specialize in the field of molecular oncology diagnostics, with a deep understanding of the clinical significance and technical challenges of targets like EGFR vIII. Our products are designed to address the core needs of researchers.
3.Exceptional Stability and Reliability: Strict manufacturing and storage standards guarantee high stability and low degradation rates throughout the product's lifecycle, providing peace of mind for your critical experiments.
4.Comprehensive Support: We provide detailed technical documentation and have a dedicated technical support team ready to offer timely assistance with experimental design and technical challenges.
5.High Cost-Effectiveness: We are committed to providing the research community with high-quality, affordable research tools, helping you maximize your research budget for core scientific investigations.
A:This product is provided in RNA format. This allows it to directly mimic the EGFR vIII transcript extracted from clinical samples, making it suitable for detection methods that start with RNA, such as RT-PCR and RNA-Seq, ensuring highly comparable results.
A:The product must be stored at -90°C to -70°C in an ultra-low temperature freezer. For use, thaw quickly on ice and briefly centrifuge. To avoid freeze-thaw cycles, it is recommended to aliquot the product based on your typical usage volume.
A:Each product comes with a specific Certificate of Analysis (COA), which can be downloaded from the product page or via the included instructions. The COA contains key QC data such as precise concentration, purity (A260/A280 ratio), and RNA electrophoresis gel images.
A:No, it cannot. This product is clearly labeled "For Research Use Only" and is not intended for use in clinical diagnostic procedures. Clinical diagnostics require products approved by relevant regulatory bodies (e.g., FDA, CE-IVD) with appropriate certifications.
Q5:Which detection platforms is this standard suitable for?
A:This standard has broad applications and is suitable for any detection platform that uses RNA as a template, including but not limited to RT-qPCR, digital PCR (dPCR), NGS (especially RNA-Seq), and NanoString. It is an ideal tool for validating the ability of these platforms to detect EGFR vIII.
Q6:How is RNA stability ensured during shipping?
A:We ship the product using high-quality insulated packaging with dry ice to ensure it remains frozen throughout the entire transit process, guaranteeing you receive a product with optimal activity and quality.
Product Information | AI-Edigene® EGFR VIII(E2-E7 Del) Fusion |
| Catalog No. | CBP20179R |
Format | RNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | -90~-70°C |
Expiry | 12 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® EGFR VIII(E2-E7 Del) Fusion |
Left Gene | EGFR |
Left Breakpoint | chr7:55087058:+ |
Right Gene | EGFR |
Right Breakpoint | chr7:55223523:+ |
Cosmic ID | N/A |
Buffer | RNase-free H2O |
Related product list
Product Name | Catalog No. |
AI-Edigene® EGFR VIII (E2-E7del) Translocation | CBP20203D# |
AI-Edigene® EGFR(E25)-SEPT14(E10) Fusion | CBP20233R |
AI-Edigene® EGFR(E24)-SEPT14(E10) Fusion | CBP20234R |
AI-Edigene® EGFR(E24)-PSPH(E8) Fusion | CBP20235R |
If you have any questions, please feel free to contact us at sales@cb-gene.com
Product Overview
The AI-Edigene® EGFR VIII(E2-E7 Del) Fusion is a rigorously designed and quality-controlled, in vitro transcribed RNA reference material that accurately mimics the transcript of the naturally occurring EGFR vIII fusion gene in humans. This variant is formed by an in-frame deletion of exons 2 through 7 (E2-E7) of the EGFR gene, resulting in a protein lacking 267 amino acids in the extracellular domain, which leads to constitutive, ligand-independent oncogenic activation.
Technical Specifications
Format: High-purity RNA
Fusion Gene: EGFR-EGFR (E2-E7 Del)
Genomic Coordinates: Left Breakpoint chr7:55087058:+; Right Breakpoint chr7:55223523:+
Gene Background: Human EGFR gene
Buffer: RNase-free H2O, maximizing RNA integrity
Purity & Concentration: Detailed Certificate of Analysis (COA) provided for traceability
Storage Conditions: -90°C ~ -70°C (Ultra-low temperature freezer)
Shelf Life: 12 months from the date of manufacture
Intended Use: For Research Use Only. Not for use in diagnostic procedures.
With its clearly defined genomic breakpoints, this product serves as a molecular "ruler" for your experiments, forming the foundation for precise qualitative and quantitative analysis.
The sequence is strictly designed according to the known EGFR vIII (E2-E7 Del) sequence, with defined breakpoints (chr7:55087058:+ and chr7:55223523:+). It is verified by Sanger sequencing, ensuring high concordance with the variant sequence found in actual clinical samples.
The product undergoes stringent purification to remove impurities and enzyme residues. RNA electrophoresis confirms excellent integrity with no signs of degradation, allowing direct use in downstream experiments and guaranteeing reliable results.
Preserved in RNase-free water and stored at -90°C ~ -70°C, it is effectively protected from RNA degradation, ensuring consistent performance and low inter-batch variation throughout its shelf life.
Ready-to-Use Design
Upon receipt, users can simply dilute or aliquot the product as needed for their experiments, saving significant time and cost associated with in-house preparation.
Accurate Quantification
The product is supplied with precise concentration data (see COA), supporting absolute quantification analysis, such as generating standard curves for dPCR or NGS platforms.
When used as a positive control during the development of RT-PCR, ddPCR, or NGS panels, it helps optimize reaction conditions, ensuring the assay specifically identifies the EGFR vIII fusion and avoids false-negative or false-positive results.
Using the same standard across different experiment batches allows for effective monitoring of process stability and evaluation of operator performance and reagent quality, ensuring long-term comparability and reproducibility of data.
Accelerates Assay Development and Validation
For newly established detection methods, this standard acts as a "gold standard" to verify success and reliability, significantly shortening the development-to-application timeline.
Facilitates Cross-Platform Comparison
It can be used as a universal reference for comparing results and standardizing data across different technological platforms, such as qPCR, dPCR, and NGS, promoting multi-center research collaboration.
Eliminates Risk of Sample Cross-Contamination
Using a synthetic standard instead of patient-derived material completely avoids biosafety risks and the potential for cross-contamination between samples, ensuring safer and more ethically compliant operations.
Assay Development & Optimization: For developing and optimizing detection schemes for EGFR vIII using RT-qPCR, ddPCR, NanoString, or NGS.
Internal Quality Control (IQC): Serves as a positive control and quantitative standard for each assay run, monitoring the stability of the entire workflow (from RNA extraction to data analysis).
Kit Performance Evaluation: Used to evaluate the sensitivity, specificity, and limit of detection (LoD) of commercial EGFR mutation detection kits.
Laboratory Proficiency Testing & Standardization: Acts as a third-party quality control material for inter-laboratory proficiency testing and assay harmonization.
Basic Mechanism Research: In cell model studies, it can be used as a template for transfection to study the biological functions of EGFR vIII and its impact on drug responses.
1.Precision at Source, Superior Quality: We control quality from the source, ensuring accuracy at the single-base level. Multiple QC techniques, including electrophoresis and sequencing, are employed, and all key data are provided via a downloadable COA for full transparency.
2.Specialization and Deep Expertise: We specialize in the field of molecular oncology diagnostics, with a deep understanding of the clinical significance and technical challenges of targets like EGFR vIII. Our products are designed to address the core needs of researchers.
3.Exceptional Stability and Reliability: Strict manufacturing and storage standards guarantee high stability and low degradation rates throughout the product's lifecycle, providing peace of mind for your critical experiments.
4.Comprehensive Support: We provide detailed technical documentation and have a dedicated technical support team ready to offer timely assistance with experimental design and technical challenges.
5.High Cost-Effectiveness: We are committed to providing the research community with high-quality, affordable research tools, helping you maximize your research budget for core scientific investigations.
A:This product is provided in RNA format. This allows it to directly mimic the EGFR vIII transcript extracted from clinical samples, making it suitable for detection methods that start with RNA, such as RT-PCR and RNA-Seq, ensuring highly comparable results.
A:The product must be stored at -90°C to -70°C in an ultra-low temperature freezer. For use, thaw quickly on ice and briefly centrifuge. To avoid freeze-thaw cycles, it is recommended to aliquot the product based on your typical usage volume.
A:Each product comes with a specific Certificate of Analysis (COA), which can be downloaded from the product page or via the included instructions. The COA contains key QC data such as precise concentration, purity (A260/A280 ratio), and RNA electrophoresis gel images.
A:No, it cannot. This product is clearly labeled "For Research Use Only" and is not intended for use in clinical diagnostic procedures. Clinical diagnostics require products approved by relevant regulatory bodies (e.g., FDA, CE-IVD) with appropriate certifications.
Q5:Which detection platforms is this standard suitable for?
A:This standard has broad applications and is suitable for any detection platform that uses RNA as a template, including but not limited to RT-qPCR, digital PCR (dPCR), NGS (especially RNA-Seq), and NanoString. It is an ideal tool for validating the ability of these platforms to detect EGFR vIII.
Q6:How is RNA stability ensured during shipping?
A:We ship the product using high-quality insulated packaging with dry ice to ensure it remains frozen throughout the entire transit process, guaranteeing you receive a product with optimal activity and quality.
Product Information | AI-Edigene® EGFR VIII(E2-E7 Del) Fusion |
| Catalog No. | CBP20179R |
Format | RNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | -90~-70°C |
Expiry | 12 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® EGFR VIII(E2-E7 Del) Fusion |
Left Gene | EGFR |
Left Breakpoint | chr7:55087058:+ |
Right Gene | EGFR |
Right Breakpoint | chr7:55223523:+ |
Cosmic ID | N/A |
Buffer | RNase-free H2O |
Related product list
Product Name | Catalog No. |
AI-Edigene® EGFR VIII (E2-E7del) Translocation | CBP20203D# |
AI-Edigene® EGFR(E25)-SEPT14(E10) Fusion | CBP20233R |
AI-Edigene® EGFR(E24)-SEPT14(E10) Fusion | CBP20234R |
AI-Edigene® EGFR(E24)-PSPH(E8) Fusion | CBP20235R |
If you have any questions, please feel free to contact us at sales@cb-gene.com
