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CBP40048
CBP40048
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Product Overview
The BRCA2 Loss Reference Standard is a high-quality genomic DNA reference material specifically developed for oncology research and molecular diagnostics. This standard mimics the genetic state of BRCA2 gene monoallelic loss (Copy Number = 1), which occurs in various cancers.
The BRCA2 gene, Breast Cancer Type 2 Susceptibility Protein, is a crucial tumor suppressor gene. The protein it encodes plays a central role in the Homologous Recombination pathway for repairing DNA double-strand breaks, acting as a guardian of genome stability (PMID: 27530658). When BRCA2 function is lost, cells are more prone to accumulate genetic mutations, leading to carcinogenesis.
Hereditary Context: Germline mutations in BRCA2 significantly increase an individual's lifetime risk of developing breast cancer, ovarian cancer, and other malignancies (PMID: 23364291).
Somatic Context: Somatic mutations in BRCA2 are most frequently found in solid tumors such as colon cancer, non-small cell lung cancer (NSCLC), and ovarian cancer (PMID: 27283171).
Therefore, accurately detecting the genetic status of BRCA2 (e.g., loss, mutation) is of critical clinical importance for cancer risk assessment, prognosis determination, and guiding targeted therapies (such as PARP inhibitors). This BRCA2 Loss Reference Standard was born to standardize and validate the accuracy of such detection.
We understand that the quality of a reference standard is the lifeline of reliable scientific data. Therefore, we subject every batch of the BRCA2 Loss Reference Standard to extremely stringent quality control:
Clearly Defined Genetic Alteration:
The standard is unambiguously defined as BRCA2 Gene Loss, with a copy number of 1, accurately simulating the haploinsufficiency state commonly found in clinical samples.
High-Purity Genomic DNA:
The product consists of high-purity genomic DNA, suitable for various downstream molecular biology applications.
Comprehensive QC Data:
We provide downloadable Certificates of Analysis (COA) for full data transparency and traceability.
Concentration & Purity: Provides precise concentration (download COA) and purity (e.g., A260/A280 ratio) data.
DNA Electrophoresis: DNA integrity and absence of degradation are verified via electrophoregram (download COA).
Sanger Sequencing:Key regions of the BRCA2 gene are verified by Sanger sequencing (download COA), ensuring a clear sequence background.
Stable Storage & Long Shelf-Life:
The product should be stored at 2-8°C and has a shelf life of 36 months from the date of manufacture, providing stable support for your long-term research projects.
Reliable & Reproducible Results: Serving as a "gold standard" for your experiments, it effectively monitors the entire workflow from DNA extraction to result analysis. It ensures high consistency and comparability of results across different batches and laboratories, significantly enhancing the credibility of your research findings.
Enhanced Assay Sensitivity & Specificity: When developing detection methods like NGS (Next-Generation Sequencing) or qPCR, using this standard helps accurately set thresholds for calling gene loss (e.g., log2 ratio in CNV analysis), effectively distinguishing true positives from false positives/negatives.
Accelerated R&D Pipeline: In the development of companion diagnostic kits and anticancer drugs (especially PARP inhibitors), using a certified reference standard can significantly reduce the time required for assay development and validation, accelerating product registration and time-to-market.
Cost Efficiency: By identifying and correcting biases in the detection system early on, it prevents the need for experiment repetition, sample waste, and time loss due to inaccurate results, leading to significant long-term cost savings in R&D.
The BRCA2 Loss Reference Standard is a versatile reference material widely applicable in the following fields:
Cancer Molecular Biology Research: Used to study the role of BRCA2 loss in tumor initiation, progression, and metastasis.
Companion Diagnostic Kit Development: Serves as a positive control for developing and validating commercial kits designed to detect BRCA2 copy number variations.
Clinical Testing Laboratory QC: Used as an internal quality control material for daily monitoring of assay stability and accuracy, supporting compliance with laboratory accreditation standards (e.g., CAP/CLIA).
Drug Discovery & Biomarker Exploration: Used in clinical trials for targeted therapies (e.g., PARP inhibitors) to screen appropriate patient populations (biomarker-positive) and evaluate efficacy.
Technology Platform Performance Evaluation: Used to assess and compare the performance of different genetic testing platforms (e.g., NGS panels, microarrays, FISH) in detecting BRCA2 copy number variations.
Precise Definition:We provide not just DNA, but a standard with a molecularly defined "BRCA2 Loss" status, offering complete information and clear value.
Rigorous QC Protocol: Each batch comes with a detailed COA, including concentration, purity, integrity, and sequencing verification, ensuring transparent quality and your peace of mind .
Excellent Stability:The 36-month shelf life and simple 2-8°C storage condition allow for convenient access and easy planning of long-term experiments .
Strong Scientific Foundationl: The product is designed based on the latest research (citing high-impact PMID references) regarding BRCA2's role in DNA repair and cancer, ensuring its scientific relevance and clinical correlation.
Focus on Research Applications: We are dedicated to providing high-quality "Research Use Only" reference standards for the scientific community, understanding researchers' core needs, and designing products that fit real-world application scenarios.
Yes, absolutely. This product is high-purity genomic DNA, making it an ideal starting material for NGS library construction, particularly for sequencing protocols targeting copy number variation (CNV) detection
No, it cannot. This product is explicitly labeled "For Research Use Only. Not for use in diagnostic procedures." It is intended to support In Vitro Diagnostic (IVD) kit development, laboratory internal quality control, and basic scientific research.
You can download the complete Certificate of Analysis (COA) provided with the product. It contains the primary data from Sanger sequencing, electrophoresis, etc., which you can review. We also encourage users to validate the standard on their own detection platform upon first use to establish baseline data.
The product is shipped at 2-8°C to ensure stability. Upon receipt, please store it immediately in a 2-8°C refrigerator. Avoid repeated freeze-thaw cycles; aliquoting upon first use is recommended.
Is this standard derived from a cell line or synthetically produced?
To protect donor privacy and commercial confidentiality, the specific source is typically not disclosed. However, we guarantee that this reference standard has undergone a series of rigorous technical validations (e.g., Sanger sequencing, copy number analysis), and its BRCA2 loss genotype is clearly defined and stable, fully meeting the technical requirements for a reference standard.
Product Overview
The BRCA2 Loss Reference Standard is a high-quality genomic DNA reference material specifically developed for oncology research and molecular diagnostics. This standard mimics the genetic state of BRCA2 gene monoallelic loss (Copy Number = 1), which occurs in various cancers.
The BRCA2 gene, Breast Cancer Type 2 Susceptibility Protein, is a crucial tumor suppressor gene. The protein it encodes plays a central role in the Homologous Recombination pathway for repairing DNA double-strand breaks, acting as a guardian of genome stability (PMID: 27530658). When BRCA2 function is lost, cells are more prone to accumulate genetic mutations, leading to carcinogenesis.
Hereditary Context: Germline mutations in BRCA2 significantly increase an individual's lifetime risk of developing breast cancer, ovarian cancer, and other malignancies (PMID: 23364291).
Somatic Context: Somatic mutations in BRCA2 are most frequently found in solid tumors such as colon cancer, non-small cell lung cancer (NSCLC), and ovarian cancer (PMID: 27283171).
Therefore, accurately detecting the genetic status of BRCA2 (e.g., loss, mutation) is of critical clinical importance for cancer risk assessment, prognosis determination, and guiding targeted therapies (such as PARP inhibitors). This BRCA2 Loss Reference Standard was born to standardize and validate the accuracy of such detection.
We understand that the quality of a reference standard is the lifeline of reliable scientific data. Therefore, we subject every batch of the BRCA2 Loss Reference Standard to extremely stringent quality control:
Clearly Defined Genetic Alteration:
The standard is unambiguously defined as BRCA2 Gene Loss, with a copy number of 1, accurately simulating the haploinsufficiency state commonly found in clinical samples.
High-Purity Genomic DNA:
The product consists of high-purity genomic DNA, suitable for various downstream molecular biology applications.
Comprehensive QC Data:
We provide downloadable Certificates of Analysis (COA) for full data transparency and traceability.
Concentration & Purity: Provides precise concentration (download COA) and purity (e.g., A260/A280 ratio) data.
DNA Electrophoresis: DNA integrity and absence of degradation are verified via electrophoregram (download COA).
Sanger Sequencing:Key regions of the BRCA2 gene are verified by Sanger sequencing (download COA), ensuring a clear sequence background.
Stable Storage & Long Shelf-Life:
The product should be stored at 2-8°C and has a shelf life of 36 months from the date of manufacture, providing stable support for your long-term research projects.
Reliable & Reproducible Results: Serving as a "gold standard" for your experiments, it effectively monitors the entire workflow from DNA extraction to result analysis. It ensures high consistency and comparability of results across different batches and laboratories, significantly enhancing the credibility of your research findings.
Enhanced Assay Sensitivity & Specificity: When developing detection methods like NGS (Next-Generation Sequencing) or qPCR, using this standard helps accurately set thresholds for calling gene loss (e.g., log2 ratio in CNV analysis), effectively distinguishing true positives from false positives/negatives.
Accelerated R&D Pipeline: In the development of companion diagnostic kits and anticancer drugs (especially PARP inhibitors), using a certified reference standard can significantly reduce the time required for assay development and validation, accelerating product registration and time-to-market.
Cost Efficiency: By identifying and correcting biases in the detection system early on, it prevents the need for experiment repetition, sample waste, and time loss due to inaccurate results, leading to significant long-term cost savings in R&D.
The BRCA2 Loss Reference Standard is a versatile reference material widely applicable in the following fields:
Cancer Molecular Biology Research: Used to study the role of BRCA2 loss in tumor initiation, progression, and metastasis.
Companion Diagnostic Kit Development: Serves as a positive control for developing and validating commercial kits designed to detect BRCA2 copy number variations.
Clinical Testing Laboratory QC: Used as an internal quality control material for daily monitoring of assay stability and accuracy, supporting compliance with laboratory accreditation standards (e.g., CAP/CLIA).
Drug Discovery & Biomarker Exploration: Used in clinical trials for targeted therapies (e.g., PARP inhibitors) to screen appropriate patient populations (biomarker-positive) and evaluate efficacy.
Technology Platform Performance Evaluation: Used to assess and compare the performance of different genetic testing platforms (e.g., NGS panels, microarrays, FISH) in detecting BRCA2 copy number variations.
Precise Definition:We provide not just DNA, but a standard with a molecularly defined "BRCA2 Loss" status, offering complete information and clear value.
Rigorous QC Protocol: Each batch comes with a detailed COA, including concentration, purity, integrity, and sequencing verification, ensuring transparent quality and your peace of mind .
Excellent Stability:The 36-month shelf life and simple 2-8°C storage condition allow for convenient access and easy planning of long-term experiments .
Strong Scientific Foundationl: The product is designed based on the latest research (citing high-impact PMID references) regarding BRCA2's role in DNA repair and cancer, ensuring its scientific relevance and clinical correlation.
Focus on Research Applications: We are dedicated to providing high-quality "Research Use Only" reference standards for the scientific community, understanding researchers' core needs, and designing products that fit real-world application scenarios.
Yes, absolutely. This product is high-purity genomic DNA, making it an ideal starting material for NGS library construction, particularly for sequencing protocols targeting copy number variation (CNV) detection
No, it cannot. This product is explicitly labeled "For Research Use Only. Not for use in diagnostic procedures." It is intended to support In Vitro Diagnostic (IVD) kit development, laboratory internal quality control, and basic scientific research.
You can download the complete Certificate of Analysis (COA) provided with the product. It contains the primary data from Sanger sequencing, electrophoresis, etc., which you can review. We also encourage users to validate the standard on their own detection platform upon first use to establish baseline data.
The product is shipped at 2-8°C to ensure stability. Upon receipt, please store it immediately in a 2-8°C refrigerator. Avoid repeated freeze-thaw cycles; aliquoting upon first use is recommended.
Is this standard derived from a cell line or synthetically produced?
To protect donor privacy and commercial confidentiality, the specific source is typically not disclosed. However, we guarantee that this reference standard has undergone a series of rigorous technical validations (e.g., Sanger sequencing, copy number analysis), and its BRCA2 loss genotype is clearly defined and stable, fully meeting the technical requirements for a reference standard.
Related product list
Name | Catalog No |
BRCA1 Amplification Reference Standard | CBP40051 |
BRCA1 Loss Reference Standard | CBP40031 |
BRCA2 Amplification Reference Standard | CBP40052 |
| BRCA2 Loss (CN=1) Reference Standard (Pair-P19) | CBP40129 |
Related product list
Name | Catalog No |
BRCA1 Amplification Reference Standard | CBP40051 |
BRCA1 Loss Reference Standard | CBP40031 |
BRCA2 Amplification Reference Standard | CBP40052 |
| BRCA2 Loss (CN=1) Reference Standard (Pair-P19) | CBP40129 |
