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Product Overview
The AI-Edigene® BRAF p.K601E Reference Standard Plus is a rigorously validated, genomic DNA (gDNA) reference standard intended for Research Use Only. This product contains a homozygous mutation at the BRAF c.1801A>G locus, resulting in the substitution of lysine (K) for glutamic acid (E) at amino acid position 601 in the encoded serine/threonine-protein kinase B-Raf—the p.K601E mutation.
The BRAF protein is a key member of the Raf family, transmitting signals through the MAP kinase pathway to regulate cell proliferation and growth. BRAF mutations, particularly specific missense mutations like p.K601E, are strongly associated with the pathogenesis and drug resistance of numerous cancers. This standard is designed to provide a stable and homogeneous reference material for the development, validation, calibration, and routine quality control of BRAF p.K601E detection methods (e.g., Next-Generation Sequencing, Sanger Sequencing, digital PCR).
DNA Change: c.1801A>G
Amino Acid Change: p.Lys601Glu (p.K601E)
Mutation Type: Missense Substitution
Zygosity: Homozygous, ensuring a clear and strong mutant signal in assays.
Precise Characterization and Traceability:
Allelic Frequency: 100%, providing an ideal benchmark for assessing assay sensitivity and specificity.
Standard Transcript: Based on ENST00000288602, consistent with major databases and clinical guidelines.
Authoritative Database ID: COSMIC ID (COSM478), enabling easy cross-referencing within the internationally recognized cancer mutation database.
Precise Genomic Location: Mapped to chr7:140453134 based on the GRCh37 (hg19) reference genome.
High-Purity DNA: The product is purified genomic DNA supplied in Tris-EDTA buffer, ensuring excellent stability and ready-to-use convenience for downstream applications.
Rigorous Quality Control: Each batch is verified by both DNA electrophoresis and Sanger sequencing, guaranteeing sequence accuracy and integrity. Users can download the Certificate of Analysis (COA) for detailed concentration, purity, and QC data.
Guaranteed Stability: Recommended storage at 2-8°C with a 36-month shelf life, supporting your long-term experimental plans.
Enhanced Assay Reliability: Serves as a perfect positive control to rule out false-negative or false-positive results caused by reagents, instruments, or operational procedures, ensuring the robustness of your detection method.
Facilitates Inter-laboratory Standardization: Using a unified reference standard helps align results across different laboratories and platforms, promoting data integration in multi-center studies.
Optimizes Limit of Detection (LOD): The homozygous genotype makes it an excellent tool for evaluating the sensitivity of detection methods, particularly for assessing low-frequency variant detection capabilities.
Streamlines Workflow: The ready-to-use gDNA format eliminates the need for tedious cell culture or DNA extraction steps, saving valuable research time.
Powerful Traceability: Links to COSMIC and standard transcripts ensure your experimental data is internationally comparable, strengthening the credibility of your research findings.
This product is strictly for Research Use Only (RUO) and is primarily applied in the following areas:
Companion Diagnostic Development & Validation: Used for performance parameter confirmation during the R&D phase of IVD test kits.
Laboratory Developed Test (LDT) Validation: Provides calibration and quality control for hospitals and clinical laboratories establishing LDTs such as NGS, qPCR, and dPCR.
Detection Platform Performance Evaluation: Used to assess and compare the accuracy and precision of different sequencing platforms or detection technologies.
Quality Assurance & Proficiency Testing: Serves as an internal control for continuous monitoring of laboratory testing performance.
Basic Research in Cancer Molecular Biology: Used to investigate the specific role of the BRAF p.K601E mutation in tumorigenesis, progression, and drug resistance mechanisms.
Expertise and Focus: Edigene specializes in the field of molecular diagnostic standards, with a deep understanding of the importance of accurate detection for clinical and research applications.
Rigorous Quality Control System: Every step, from raw material selection to the final product, undergoes strict QC verification, supported by a traceable COA for your peace of mind.
Technological Foresight: Product design keeps pace with industry guidelines and database updates (e.g., GRCh37/hg19), ensuring compatibility with mainstream bioinformatics pipelines.
Excellent User Experience: Clear product information, easy access to COA downloads, stable product supply, and comprehensive customer support to fully enable your research.
This product is Homozygous, with a 100% allele frequency. This means all DNA molecules carry the c.1801A>G mutation at this locus.
It is widely applicable to various DNA-based mutation detection technologies, including but not limited to Sanger Sequencing, Next-Generation Sequencing (NGS), digital PCR (dPCR), ARMS-PCR, and Pyrosequencing. It is an ideal tool for validating these technologies' ability to detect the BRAF p.K601E mutation.
The product is supplied as Genomic DNA dissolved in Tris-EDTA Buffer. Upon receipt, briefly centrifuge the tube and store it at 2-8°C. Avoid repeated freeze-thaw cycles to maintain DNA integrity.
No. This product is clearly labeled "For Research Use Only" and is not intended for use in clinical diagnosis, therapeutic decision-making, or any other clinical applications.
Where can I find the product's concentration and quality control data?
Each vial comes with a dedicated Certificate of Analysis (COA). You can download the corresponding COA using the information on the product packaging or by contacting our sales team. The COA details the batch-specific concentration, purity (A260/A280 ratio), and QC results from DNA electrophoresis and sequencing.
Product Information | AI-Edigene® BRAF p.K601E Reference Standard Plus |
| Catalog No. | CBP10428 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® BRAF p.K601E Reference Standard Plus |
DNA Change | c.1801A>G |
AA Change | p.K601E |
Mutation type | Substitution - Missense |
Zygosity | Homozygous |
Allelic Frequency | 100% |
| Transcript | ENST00000288602 |
Cosmic ID | COSM478 |
Chr position(GRCh37) | chr7:140453134 |
Buffer | Tris-EDTA |
Related product list
Name | Catalog No. | Details |
AI-Edigene® BRAF p.K601E Reference Standard Plus | CBP10428 | |
AI-Edigene® BRAF p.V600D Reference Standard Plus | CBP10423 | |
AI-Edigene® BRAF p.V600E Reference Standard Plus | CBP10424 | |
AI-Edigene® BRAF p.V600G Reference Standard Plus | CBP10427 | |
AI-Edigene® BRAF p.V600K Reference Standard Plus | CBP10413 |  COA Download：CBP10413 GBD42749765 |
AI-Edigene® BRAF p.V600M Reference Standard Plus | CBP10425 | COA Download：CBP10425 CGFA23050618 |
AI-Edigene® BRAF p.V600R Reference Standard Plus | CBP10426 | COA Download：CBP10426 CGFA23040403 |
BRAF p.G464V Reference Standard | CBP10695 | COA Download：CBP10695 CBFA23092001 |
BRAF p.G466V Reference Standard | CBP10239 | COA Download：CBP10239 CGFA23041105 |
BRAF p.G469A Reference Standard | CBP10235 | |
BRAF p.G469R Reference Standard | CBP10236 | |
BRAF p.G596R Reference Standard | CBP10242 | COA Download：CBP10242 CBFA22101479 |
BRAF p.K601N Reference Standard | CBP10237 | COA Download：CBP10237 CBFA23062104 |
BRAF p.L597R Reference Standard | CBP10240 | |
BRAF p.L597V Reference Standard | CBP10241 | |
BRAF p.N581Y Reference Standard | CBP10238 | |
BRAF p.V600E Reference Standard | CBP10234 | COA Download：CBP10234 CBFA24120122X |
BRAF p.G464E Reference Standard | CBP10697 |
If you have any questions, please feel free to contact us at sales@cb-gene.com
Product Overview
The AI-Edigene® BRAF p.K601E Reference Standard Plus is a rigorously validated, genomic DNA (gDNA) reference standard intended for Research Use Only. This product contains a homozygous mutation at the BRAF c.1801A>G locus, resulting in the substitution of lysine (K) for glutamic acid (E) at amino acid position 601 in the encoded serine/threonine-protein kinase B-Raf—the p.K601E mutation.
The BRAF protein is a key member of the Raf family, transmitting signals through the MAP kinase pathway to regulate cell proliferation and growth. BRAF mutations, particularly specific missense mutations like p.K601E, are strongly associated with the pathogenesis and drug resistance of numerous cancers. This standard is designed to provide a stable and homogeneous reference material for the development, validation, calibration, and routine quality control of BRAF p.K601E detection methods (e.g., Next-Generation Sequencing, Sanger Sequencing, digital PCR).
DNA Change: c.1801A>G
Amino Acid Change: p.Lys601Glu (p.K601E)
Mutation Type: Missense Substitution
Zygosity: Homozygous, ensuring a clear and strong mutant signal in assays.
Precise Characterization and Traceability:
Allelic Frequency: 100%, providing an ideal benchmark for assessing assay sensitivity and specificity.
Standard Transcript: Based on ENST00000288602, consistent with major databases and clinical guidelines.
Authoritative Database ID: COSMIC ID (COSM478), enabling easy cross-referencing within the internationally recognized cancer mutation database.
Precise Genomic Location: Mapped to chr7:140453134 based on the GRCh37 (hg19) reference genome.
High-Purity DNA: The product is purified genomic DNA supplied in Tris-EDTA buffer, ensuring excellent stability and ready-to-use convenience for downstream applications.
Rigorous Quality Control: Each batch is verified by both DNA electrophoresis and Sanger sequencing, guaranteeing sequence accuracy and integrity. Users can download the Certificate of Analysis (COA) for detailed concentration, purity, and QC data.
Guaranteed Stability: Recommended storage at 2-8°C with a 36-month shelf life, supporting your long-term experimental plans.
Enhanced Assay Reliability: Serves as a perfect positive control to rule out false-negative or false-positive results caused by reagents, instruments, or operational procedures, ensuring the robustness of your detection method.
Facilitates Inter-laboratory Standardization: Using a unified reference standard helps align results across different laboratories and platforms, promoting data integration in multi-center studies.
Optimizes Limit of Detection (LOD): The homozygous genotype makes it an excellent tool for evaluating the sensitivity of detection methods, particularly for assessing low-frequency variant detection capabilities.
Streamlines Workflow: The ready-to-use gDNA format eliminates the need for tedious cell culture or DNA extraction steps, saving valuable research time.
Powerful Traceability: Links to COSMIC and standard transcripts ensure your experimental data is internationally comparable, strengthening the credibility of your research findings.
This product is strictly for Research Use Only (RUO) and is primarily applied in the following areas:
Companion Diagnostic Development & Validation: Used for performance parameter confirmation during the R&D phase of IVD test kits.
Laboratory Developed Test (LDT) Validation: Provides calibration and quality control for hospitals and clinical laboratories establishing LDTs such as NGS, qPCR, and dPCR.
Detection Platform Performance Evaluation: Used to assess and compare the accuracy and precision of different sequencing platforms or detection technologies.
Quality Assurance & Proficiency Testing: Serves as an internal control for continuous monitoring of laboratory testing performance.
Basic Research in Cancer Molecular Biology: Used to investigate the specific role of the BRAF p.K601E mutation in tumorigenesis, progression, and drug resistance mechanisms.
Expertise and Focus: Edigene specializes in the field of molecular diagnostic standards, with a deep understanding of the importance of accurate detection for clinical and research applications.
Rigorous Quality Control System: Every step, from raw material selection to the final product, undergoes strict QC verification, supported by a traceable COA for your peace of mind.
Technological Foresight: Product design keeps pace with industry guidelines and database updates (e.g., GRCh37/hg19), ensuring compatibility with mainstream bioinformatics pipelines.
Excellent User Experience: Clear product information, easy access to COA downloads, stable product supply, and comprehensive customer support to fully enable your research.
This product is Homozygous, with a 100% allele frequency. This means all DNA molecules carry the c.1801A>G mutation at this locus.
It is widely applicable to various DNA-based mutation detection technologies, including but not limited to Sanger Sequencing, Next-Generation Sequencing (NGS), digital PCR (dPCR), ARMS-PCR, and Pyrosequencing. It is an ideal tool for validating these technologies' ability to detect the BRAF p.K601E mutation.
The product is supplied as Genomic DNA dissolved in Tris-EDTA Buffer. Upon receipt, briefly centrifuge the tube and store it at 2-8°C. Avoid repeated freeze-thaw cycles to maintain DNA integrity.
No. This product is clearly labeled "For Research Use Only" and is not intended for use in clinical diagnosis, therapeutic decision-making, or any other clinical applications.
Where can I find the product's concentration and quality control data?
Each vial comes with a dedicated Certificate of Analysis (COA). You can download the corresponding COA using the information on the product packaging or by contacting our sales team. The COA details the batch-specific concentration, purity (A260/A280 ratio), and QC results from DNA electrophoresis and sequencing.
Product Information | AI-Edigene® BRAF p.K601E Reference Standard Plus |
| Catalog No. | CBP10428 |
Format | Genomic DNA |
Intended Use | Research Use Only |
Unit Size | 1ug |
Sanger sequencing |
|
Storage | 2~8°C |
Expiry | 36 months from the date of manufacture |
Technical Data
Product Information | AI-Edigene® BRAF p.K601E Reference Standard Plus |
DNA Change | c.1801A>G |
AA Change | p.K601E |
Mutation type | Substitution - Missense |
Zygosity | Homozygous |
Allelic Frequency | 100% |
| Transcript | ENST00000288602 |
Cosmic ID | COSM478 |
Chr position(GRCh37) | chr7:140453134 |
Buffer | Tris-EDTA |
Related product list
Name | Catalog No. | Details |
AI-Edigene® BRAF p.K601E Reference Standard Plus | CBP10428 | |
AI-Edigene® BRAF p.V600D Reference Standard Plus | CBP10423 | |
AI-Edigene® BRAF p.V600E Reference Standard Plus | CBP10424 | |
AI-Edigene® BRAF p.V600G Reference Standard Plus | CBP10427 | |
AI-Edigene® BRAF p.V600K Reference Standard Plus | CBP10413 | COA Download：CBP10413 GBD42749765 |
AI-Edigene® BRAF p.V600M Reference Standard Plus | CBP10425 | COA Download：CBP10425 CGFA23050618 |
AI-Edigene® BRAF p.V600R Reference Standard Plus | CBP10426 | COA Download：CBP10426 CGFA23040403 |
BRAF p.G464V Reference Standard | CBP10695 | COA Download：CBP10695 CBFA23092001 |
BRAF p.G466V Reference Standard | CBP10239 | COA Download：CBP10239 CGFA23041105 |
BRAF p.G469A Reference Standard | CBP10235 | |
BRAF p.G469R Reference Standard | CBP10236 | |
BRAF p.G596R Reference Standard | CBP10242 | COA Download：CBP10242 CBFA22101479 |
BRAF p.K601N Reference Standard | CBP10237 | COA Download：CBP10237 CBFA23062104 |
BRAF p.L597R Reference Standard | CBP10240 | |
BRAF p.L597V Reference Standard | CBP10241 | |
BRAF p.N581Y Reference Standard | CBP10238 | |
BRAF p.V600E Reference Standard | CBP10234 | COA Download：CBP10234 CBFA24120122X |
BRAF p.G464E Reference Standard | CBP10697 |
If you have any questions, please feel free to contact us at sales@cb-gene.com
