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10% -30% STR Reference Standard

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At present, the most widely used STR is cell authentication. In 2011, American National Standards Institute issued a special cell STR identification standard, and ChP (People's Republic of China (PRC) Pharmacopoeia) also stipulated the STR test for cell line quality control. STR genotyping has been used as the gold standard in cell identification by authoritative organizations such as ICLAC and ATCC.
 
  • CBPO0005-CBPO0007

  • CBPO0005-CBPO0007

Availability:


Description

In the STR experiment, how to judge the accuracy of the results is essential. CB-Gene now introduces STR-related standards, which mainly include 1. Cell identification: autosomal series and sex chromosome series, as well as species pollution. 2.Reproductive inheritance: chromosome aneuploid pairing standard (mother and fetus) and maternal cell pollution detection standard.

The proportion is the quality proportion:10% mixed sample ,20% mixed sample and 30% mixed sample.


General information

Name

10% STR Reference Standard

20% STR Reference Standard

30% STR Reference Standard

Cat. No.

CBPO0005

CBPO0006

CBPO0007

Format

Genomic DNA

Genomic DNA

Genomic DNA

Unit Size

1ug

1ug

1ug

Buffer

TE Buffer

TE Buffer

TE Buffer

Concentration

Download for COA

Download for COA

Download for COA

Purofication

Download for COA

Download for COA

Download for COA

DNA Electrophoresis

Download for COA

Download for COA

Download for COA

Storage Conditions

2~8℃

2~8℃

2~8℃

Expiry

36 months from the date of manufacture

36 months from the date of manufacture

36 months from the date of manufacture


Technical Data


Sample A Sample B
CBPO0005 10% STR typing 90% STR typing
CBPO0006 20% STR typing 80% STR typing
CBPO0007 30% STR typing 70% STR typing
Data

Allele

Data

Allele

Data

Allele

Data

Allele

Data

CSF1PO

10,12

D18S51

15,19

CSF1PO

10,11, or

D18S51

15,18

10,11,12*

D2S1338

19,23

D19S433

14,15

D2S1338

23,23

D19S433

13,14

D2S441

10,14

D21S11

30,30

D2S441

11,12

D21S11

29,30

D3S1358

14,15

FGA

23,24

D3S1358

15,17

FGA

24,26

D5S818

11,11

Penta D

12,12

D5S818

11,13

Penta D

8,12

D6S1043

12,18

Penta E

12,13

D6S1043

12,12

Penta E

11,11

D7S820

10,11

TH01

8,9.3

D7S820

11,11

TH01

6,9.3

D8S1179

13,13

TPOX

8,8

D8S1179

12,13

TPOX

8,9

D12S391

18,20

vWA

17,18

D12S391

18,24

vWA

17,17

D13S317

11,11

Amel

X,X

D13S317

11,11

Amel

X,Y

D16S539

11,12



D16S539

11,11



Remark*: The relative intensity of the 12 allele is less than 10 % of the dominant 10 allele.


Product Workflow


Product development

Case Showcase

CB-Gene accurately prepare mixed gDNA samples by dPCR with different proportions of cell contamination: 10% contaminated samples, 20% contaminated samples and 30% contaminated samples, and sent the above samples to three testing companies (marked as A, B, and C) and tested them using their CE method



Technical Data


Sample ASample B
CBPO000510% STR typing90% STR typing
CBPO000620% STR typing80% STR typing
CBPO000730% STR typing70% STR typing
Data

Allele

Data

Allele

Data

Allele

Data

Allele

Data

CSF1PO

10,12

D18S51

15,19

CSF1PO

10,11, or

D18S51

15,18

10,11,12*

D2S1338

19,23

D19S433

14,15

D2S1338

23,23

D19S433

13,14

D2S441

10,14

D21S11

30,30

D2S441

11,12

D21S11

29,30

D3S1358

14,15

FGA

23,24

D3S1358

15,17

FGA

24,26

D5S818

11,11

Penta D

12,12

D5S818

11,13

Penta D

8,12

D6S1043

12,18

Penta E

12,13

D6S1043

12,12

Penta E

11,11

D7S820

10,11

TH01

8,9.3

D7S820

11,11

TH01

6,9.3

D8S1179

13,13

TPOX

8,8

D8S1179

12,13

TPOX

8,9

D12S391

18,20

vWA

17,18

D12S391

18,24

vWA

17,17

D13S317

11,11

Amel

X,X

D13S317

11,11

Amel

X,Y

D16S539

11,12



D16S539

11,11



Remark*: The relative intensity of the 12 allele is less than 10 % of the dominant 10 allele.


Product Workflow


Product Development


Case Showcase

CB-Gene accurately prepare mixed gDNA samples by dPCR with different proportions of cell contamination: 10% contaminated samples, 20% contaminated samples and 30% contaminated samples, and sent the above samples to three testing companies (marked as A, B, and C) and tested them using their CE method




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