Panel-Ref® RNA Fusion Cocktail Control

Product Overview

Gene fusions (e.g., EML4-ALK, BCR-ABL1) drive malignant blood cancers and solid tumors, making them critical biomarkers for targeted therapy. The Panel-Ref® RNA-Fusion Cocktail Control (CBP90001) is a high-quality QC material upgraded in 2021 to include 22 fusion sites (from 11) and FFPE slide format (replacing RNA solution) to better simulate clinical sample processing (e.g., RNA extraction). It contains 11 clinically relevant fusion transcripts, all quantified via ddPCR for accuracy.

Core Advantages

Clinical Sample Simulation with FFPE Format

The FFPE slide format replicates real-world clinical workflows (e.g., formalin fixation, RNA extraction), helping labs validate assay performance through every step—unlike liquid RNA standards.

Comprehensive Fusion Coverage

Including 11 high-impact fusions (e.g., NTRK, RET, ROS1 fusions), it supports validation of NGS/ddPCR-based fusion detection assays for lung, breast, and hematologic cancers.

ddPCR-Qualified Precision

Each fusion’s copy number is calibrated via ddPCR, ensuring accurate quantification for testing assay sensitivity (e.g., detecting low-abundance fusion transcripts) and reproducibility.

RNase-Free & Degradation-Resistant

Packaged in RNase-free buffer with high RNQ value, it maintains RNA integrity during storage and handling, avoiding false negatives from degraded samples.

Service Process

Pre-Order Consultation

We advise on fusion selection (e.g., prioritizing EML4-ALK for lung cancer research) and format (RNA solution vs. FFPE) based on your assay platform (e.g., RNA-seq, RT-ddPCR).

Secure, Temperature-Controlled Shipping

RNA is shipped on dry ice to preserve integrity; FFPE slides include handling instructions to prevent RNA degradation during processing.

Technical Guidance for Extraction & Testing

Our team provides protocols for FFPE RNA extraction, reverse transcription, and fusion detection, helping resolve issues like low yield or inconsistent results.

Copy Number Verification Support

We share ddPCR calibration data to help you cross-verify your assay’s quantification accuracy for each fusion transcript.

Why Choose This RNA Fusion Control?

Gene fusion detection requires robust QC to avoid misdiagnosing patients. This standard’s FFPE format, ddPCR validation, and comprehensive fusion coverage make it ideal for optimizing RNA-seq/ddPCR workflows, monitoring daily test performance, and ensuring reliable biomarker detection. Contact us to streamline your fusion testing QC.

There are three main mechanisms for gene fusion, as shown in the following figure:

There are three common mechanisms of gene fusion:

1) Chromosomal Translocation. As shown in Figure A above, the two segments on chromosomes 1 and 2 cross and exchange, resulting in the fusion of the light green gene on chromosome 1 and the orange gene on chromosome 2;

2) Interstitial deletion. As shown in the figure above, the segment between the orange gene and the light green gene on chromosome 3 is deleted, eventually resulting in the fusion of the two genes;

3) Chromosomal Inversion. For example, the segment between the orange gene and the dark green gene on chromosome 4 is inverted, eventually resulting in the fusion of the orange gene and the light green gene.

General information

Detailed Data